GLUT4 content decreases along with insulin resistance and high levels of inflammatory markers in rats with metabolic syndrome.
GLUT4 content decreases along with insulin resistance and high levels of inflammatory markers in rats with metabolic syndrome.
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DOI：
10.1186/1475-2840-11-100
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发表时间：
2012-08-16
影响因子：
9.3
通讯作者：
Schaan BD
中科院分区：
文献类型：
--
作者：
Leguisamo NM;Lehnen AM;Machado UF;Okamoto MM;Markoski MM;Pinto GH;Schaan BD
代谢综合征以胰岛素抵抗为特征，胰岛素抵抗与胰岛素敏感组织中GLUT 4含量密切相关。因此，我们在实验模型中评估了GLUT 4表达、胰岛素抵抗和炎症、代谢综合征的特征。用谷氨酸盐（MetS）处理自发性高血压新生大鼠（18只/组）9天，并与Wistar-Kyoto（C）和生理盐水处理的SHR（H）进行比较。观察血压、血脂、C反应蛋白（CRP）、白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）、脂联素等指标的变化。在心脏、白色脂肪组织和腓肠肌中分析GLUT 4蛋白。在3（3月龄）、6（6月龄）和9（9月龄）月龄时进行研究。与C组相比，MetS组大鼠的胰岛素抵抗程度更高（p<0.001，所有年龄），血压更高（3个月：p<0.001，6个月：p = 0.001，9个月：p = 0.015）。6个月时，MetS大鼠的CRP、IL-6和TNF-α高于H大鼠（p<0.001，所有比较），但9个月时MetS大鼠的脂联素较低（MetS：32 ± 2，H：42 ± 2，C：45 ± 2 pg/mL; p<0.001）。与C组大鼠相比，MetS组GLUT 4蛋白在3、6和9个月时分别降低（心脏：54%、50%和57%;腓肠肌：37%、56%和50%;脂肪组织：69%、61%和69%）。MSG治疗的SHR呈现所有代谢综合征特征，以及GLUT 4含量降低，这在代谢综合征的血糖稳态受损中起关键作用。
Metabolic syndrome is characterized by insulin resistance, which is closely related to GLUT4 content in insulin-sensitive tissues. Thus, we evaluated the GLUT4 expression, insulin resistance and inflammation, characteristics of the metabolic syndrome, in an experimental model. Spontaneously hypertensive neonate rats (18/group) were treated with monosodium glutamate (MetS) during 9 days, and compared with Wistar-Kyoto (C) and saline-treated SHR (H). Blood pressure (BP) and lipid levels, C-reactive protein (CRP), interleukin 6 (IL-6), TNF-α and adiponectin were evaluated. GLUT4 protein was analysed in the heart, white adipose tissue and gastrocnemius. Studies were performed at 3 (3-mo), 6 (6-mo) and 9 (9-mo) months of age. MetS rats were more insulin resistant (p<0.001, all ages) and had higher BP (3-mo: p<0.001, 6-mo: p = 0.001, 9-mo: p = 0.015) as compared to C. At 6 months, CRP, IL-6 and TNF-α were higher (p<0.001, all comparisons) in MetS rats vs H, but adiponectin was lower in MetS at 9 months (MetS: 32 ± 2, H: 42 ± 2, C: 45 ± 2 pg/mL; p<0.001). GLUT4 protein was reduced in MetS as compared to C rats at 3, 6 and 9-mo, respectively (Heart: 54%, 50% and 57%; Gastrocnemius: 37%, 56% and 50%; Adipose tissue: 69%, 61% and 69%). MSG-treated SHR presented all metabolic syndrome characteristics, as well as reduced GLUT4 content, which must play a key role in the impaired glycemic homeostasis of the metabolic syndrome.
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影响因子：
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