Threading immobilized DNA molecules through a solid-state nanopore at >100 μs per base rate.
Threading immobilized DNA molecules through a solid-state nanopore at >100 μs per base rate.
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DOI：
10.1021/nn4012434
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发表时间：
2013-07-23
期刊：
影响因子：
17.1
通讯作者：
Li, Jiali
中科院分区：
文献类型：
--
作者：
Hyun, Changbae;Kaur, Harpreet;Rollings, Ryan;Xiao, Min;Li, Jiali
关键词：
为了开发基于固态纳米孔的DNA测序技术，我们设计并构建了一种装置，该装置可以将DNA拴系探针尖端放置在固态纳米孔附近，控制DNA移动速度，并测量当DNA被捕获并从纳米孔释放时的离子电流变化。探针尖端的位置由基于音叉的反馈力传感器和纳米定位系统感测和控制。利用该装置，DNA链在氮化硅纳米孔中的移动速率大于100μs/base或小于1 nm/ms。该速率比通过使用光学镊子操纵DNA系留珠慢10倍，并且比先前报道的通过固态纳米孔的游离DNA易位慢1000倍，这提供了足够的时间分辨率以使用当今市场上可用的单通道记录电子器件读取系留DNA分子上的每个碱基。该装置可以同时测量3个信号：通过纳米孔的离子电流、尖端位置和在DNA被纳米孔捕获和释放的过程中的尖端振动幅度。我们展示了该装置用于测量λ DNA的捕获和释放距离，以及用于测量一端生物素化的λ DNA分子和两端拴在尖端的λ DNA分子的电流阻断信号的结果。
In pursuit of developing solid-state nanopore based DNA sequencing technology, we have designed and constructed an apparatus that can place a DNA tethered probe tip near a solid-state nanopore, control the DNA moving speed, and measure the ionic current change when a DNA is captured and released from a nanopore. The probe tip’s position is sensed and controlled by a tuning fork based feedback force sensor and a nanopositioning system. Using this newly constructed apparatus, a DNA strand moving rate of >100μs/base or <1nm/ms in silicon nitride nanopores has been accomplished. This rate is 10 times slower than by manipulating DNA tethered beads using optical tweezers and 1000 times slower than free DNA translocation through solid-state nanopores reported previously, which provides enough temporal resolution to read each base on a tethered DNA molecule using available single channel recording electronics on the market today. This apparatus can measure 3 signals simultaneously: ionic current through a nanopore, tip position, and tip vibrational amplitude during the process of a DNA’s capture and release by a nanopore. We show results of this apparatus for measuring λ DNA’s capture and release distances, and for current blockage signals of λ DNA molecules biotinylated with one end and with both ends tethered to a tip.
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影响因子：
13.3
作者：
Hyun, Changbae;Rollings, Ryan;Li, Jiali
通讯作者：
Li, Jiali
影响因子：
64.8
作者：
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影响因子：
3.5
作者：
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通讯作者：
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影响因子：
10.8
作者：
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通讯作者：
Li, JL
影响因子：
10.8
作者：
Fologea, D;Uplinger, J;Li, JL
通讯作者：
Li, JL