The effects of glaucoma filtering surgery on anterior chamber-associated immune deviation and contribution of lymphatic drainage in rats
The effects of glaucoma filtering surgery on anterior chamber-associated immune deviation and contribution of lymphatic drainage in rats
复制标题
青光眼滤过手术对大鼠前房相关免疫偏差的影响及淋巴引流的贡献
作者：
Lu, H.;Pan, Z. Q.;Wang, N. L.;Fu, T.;Lu, Q. J.
目的探讨青光眼滤过手术(GFS)是否会损害大鼠眼前房相关免疫偏离(ACAID)的能力，并探讨其可能机制。在滤过手术前切除颈淋巴(CLN)的动物和接受假滤过手术的动物作为对照对照组。用抗原特异性迟发型超敏反应(DTH)鉴定ACAID的诱导。前房注射FITC葡聚糖后，以CLN中FITC阳性细胞百分率表示CLN中的抗原水平。控制组和试验组之间的数据比较采用学生t检验进行统计分析。结果接受GFS的大鼠表现出抗原特异性DTH，而假滤过手术或切除CLN的大鼠未能获得抗原特异性DTH反应。在注射抗原后第1天，GFS组大鼠CLN中FITC阳性细胞百分率(平均值±SD：2.96±0.67%)显著高于对照组(1.57±0.48%)(P=0.001)，但在第3、5、7、12天无明显变化。结论GFS可抑制大鼠急性心肌梗死的发生，对CLN的抗原引流在此过程中起重要作用。提示GFS可能改变了眼部免疫状态。
PurposeTo investigate whether glaucoma filtering surgery (GFS) in rats would impair the eye's capacity to induce anterior chamber-associated immune deviation (ACAID) and assess the possible mechanism involved.MethodsRats subjected to GFS were injected with bovine serum antigen (BSA) into the anterior chamber to induce ACAID. Animals that had their cervical lymph nodes (CLNs) excised before filtering surgery and those that had sham filtering surgery served as control comparison groups. Antigen-specific delayed-type hypersensitivity (DTH) was used to identify the induction of ACAID. Antigen level in the CLNs was indicated by the percentage of FITC-positive cells in CLNs after FITC dextran was injected into the anterior chamber. Statistical analyses were performed using the student's t-test for comparison of data between control and experimental groups. A P< 0.05 was required for results to be considered statistically significant.ResultsRats undergoing GFS demonstrated antigen-specific DTH, while those in the sham filtering surgery or CLNs excised groups failed to acquire an antigen-specific DTH response. The percentage of FITC-positive cells in CLNs was significantly increased (P= 0.001) in GFS (mean±SD: 2.96±0.67%) vs controls (1.57±0.48%) at 1 day, but not at 3, 5, 7, or 12 days post-antigen injectionConclusionsGFS prevents the induction of ACAID in rats, and the antigen drainage to CLNs plays a critical role in this process. The results suggest that the ocular immune status might be altered by GFS.
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影响因子：
4.4
作者：
J. L. Benson;J. Niederkorn
通讯作者：
J. L. Benson;J. Niederkorn
影响因子：
4.4
作者：
Y. Sonoda;J. Streilein
通讯作者：
Y. Sonoda;J. Streilein
DOI：
10.1084/jem.192.10.1425
发表时间：
2000-11-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gretz JE;Norbury CC;Anderson AO;Proudfoot AE;Shaw S
通讯作者：
Shaw S
影响因子：
4.4
作者：
H. Kaplan;J. Streilein;T. Stevens
通讯作者：
H. Kaplan;J. Streilein;T. Stevens
诱导供体特异性 ACAID 可以延长“高危”眼的同种异体原位角膜移植物的存活率。
影响因子：
2
作者：
Sano,Y;Okamoto,S;Streilein,JW
通讯作者：
Streilein,JW