Characterization and functional analysis of a Relish gene from the Asian corn borer, Ostrinia furnacalis (Guenée).
Characterization and functional analysis of a Relish gene from the Asian corn borer, Ostrinia furnacalis (Guenée).
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亚洲玉米螟 Ostriniafurnacalis (Guenée) Relish 基因的特征和功能分析
DOI：
10.1002/arch.21841
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发表时间：
2021-10
影响因子：
2.2
通讯作者：
Feng C
中科院分区：
文献类型：
--
作者：
Chen K;Chen J;Tang T;Jiang H;Han Z;Wang L;Alradi MF;Lu S;Wei X;Liu X;Wei Y;Feng C
病原体诱导的宿主免疫应答降低了用于控制害虫的病原体的效力。然而，与果蝇的免疫系统相比，农业害虫的免疫系统在很大程度上没有通过功能分析得到证实。为了揭示亚洲玉米螟免疫基因的转录调控机制，我们采用cDNA末端快速扩增法（RACE）克隆了一个转录因子Relish的cDNA。该基因全长3,164 bp，编码956个氨基酸残基的蛋白质。生物信息学分析表明，Of-Relish具有一个RHD结构域、一个位于Q409和L410之间的预测切割位点、六个ANK重复序列和一个死亡结构域。Of-Relish对革兰氏阴性菌铜绿假单胞菌的表达反应比革兰氏阳性菌藤黄微球菌更快、更强。抗菌肽基因Attacin和Gloverin对感染的反应具有相似的表达模式。敲低Of-Relish导致Attacin和Gloverin mRNA水平降低，表明Attacin和Gloverin受Of-Relish调节。总之，结果表明，Of-Relish是O.铜绿假单胞菌，通过激活Attacin和Gloverin参与防御铜绿假单胞菌。
Pathogen-induced host immune responses reduce the efficacy of pathogens used to control pests. However, compared to the well-deciphered immunity system of Drosophila melanogaster, the immunity system of agricultural pests is largely unconfirmed through functional analysis. Beginning to unveil mechanisms of transcription regulation of immune genes in the Asian corn borer, Ostrinia furnacalis, we cloned the cDNA of a transcription factor Relish by rapid amplification of cDNA ends (RACE). The 3,164 bp cDNA, designated Of-Relish, encodes a 956-residue protein. Bioinformatic analysis showed that Of-Relish had an RHD domain, a predicted cleavage site between Q409 and L410, six ANK repeats, and a death domain. The Of-Relish expression responded to the Gram-negative bacteria Pseudomonas aeruginosa was sooner and stronger than to the Gram-positive Micrococcus luteus. The antimicrobial peptide genes Attacin and Gloverin had similar expression patterns in response to the infections. Knockdown of Of-Relish led to a decrease in Attacin and Gloverin mRNA levels, suggesting that Attacin and Gloverin were regulated by Of-Relish. Together, the results suggested that Of-Relish is a key component of the IMD pathway in O. furnacalis, involved in defense against P. aeruginosa through activation of Attacin and Gloverin.
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影响因子：
12.3
作者：
Gerardo NM;Altincicek B;Anselme C;Atamian H;Barribeau SM;de Vos M;Duncan EJ;Evans JD;Gabaldón T;Ghanim M;Heddi A;Kaloshian I;Latorre A;Moya A;Nakabachi A;Parker BJ;Pérez-Brocal V;Pignatelli M;Rahbé Y;Ramsey JS;Spragg CJ;Tamames J;Tamarit D;Tamborindeguy C;Vincent-Monegat C;Vilcinskas A
通讯作者：
Vilcinskas A
亚洲玉米螟 serpin-3 通过抑制原酚氧化酶激活蛋白酶来调节黑化级联
影响因子：
3.8
作者：
Chu, Yuan;Zhou, Fan;An, Chunju
通讯作者：
An, Chunju
IBS：用于生物序列呈现和可视化的插画家
DOI：
10.1093/bioinformatics/btv362
发表时间：
2015-10-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Liu W;Xie Y;Ma J;Luo X;Nie P;Zuo Z;Lahrmann U;Zhao Q;Zheng Y;Zhao Y;Xue Y;Ren J
通讯作者：
Ren J
影响因子：
56.9
作者：
GONZALEZCRESPO, S;LEVINE, M
通讯作者：
LEVINE, M
影响因子：
64.5
作者：
IP, YT;REACH, M;LEVINE, M
通讯作者：
LEVINE, M