Altered essential fatty acid metabolism and composition in rat liver, plasma, heart and brain after microalgal DHA addition to the diet.
Altered essential fatty acid metabolism and composition in rat liver, plasma, heart and brain after microalgal DHA addition to the diet.
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DOI：
10.1016/j.jnutbio.2010.06.008
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发表时间：
2011-08
影响因子：
5.6
通讯作者：
Salem, Norman, Jr.
中科院分区：
文献类型：
--
作者：
Lin, Yu Hong;Shah, Samit;Salem, Norman, Jr.
关键词：
为了研究不含其他高度不饱和脂肪酸(HUFA)的二十二碳六烯酸(DHA)对哺乳动物n-3和n-6必需脂肪酸(EFA)代谢和脂肪酸组成的影响，采用稳定同位素示踪技术，在含15%亚油酸和3%α-亚麻酸的基础日粮中添加或不添加1.3%藻类DHA，饲养8周。分别于给药后4、8、24、96、168、240、360和600h处死大鼠，灌胃含48 mg/kg重氢亚油酸和α-亚麻酸的混合油。测定了大鼠肝脏、血浆、心脏和脑中的脂肪酸组成及其代谢物的浓度随时间的变化。N-3EFA家族中的DHA、二十二碳五烯酸和二十碳五烯酸在喂食DHA组的所有器官中都显著增加，与对照组相比增加了5%到200%。与对照组相比，添加DHA组小鼠体内代谢物的积累量减少了1.5~2.5倍。综上所述，饲喂预制DHA可使大鼠各器官n-3HUFA含量显著增加，但以n-6HUFA为代价，同时也阻止了新合成的氢化最终产物的积累。这是第一个分离DHA对n-6和n-3EFA途径从头代谢影响的研究。
To investigate the effect of docosahexaenoic acid (DHA) without other highly unsaturated fatty acids (HUFA) on n-3 and n-6 essential fatty acid (EFA) metabolism and fatty acid composition in mammals, a stable isotope tracer technique was used in adult rats fed diets with or without 1.3% of algal DHA in a base diet containing 15% of linoleic acid and 3% of alpha-linolenic acid over 8 weeks. The rats were administered orally a mixed oil containing 48 mg/kg body weight of deuterated linoleic and alpha-linolenic acids and euthanized at 4, 8, 24, 96, 168, 240, 360 and 600 h after administration of the isotopes. Fatty acid compositions and the concentrations of deuterated precursors and their respective metabolites were determined in rat liver, plasma, heart and brain as a function of time. DHA, docosapentaenoic acid and eicosapentaenoic acid in the n-3 EFA family were significantly increased in all organs tested in the DHA-fed group, ranging from 5 to 200% greater in comparison with the control group. The accumulation of the metabolites, deuterated-DHA and deuterated-docosapentaenoic acid n-6 were greatly decreased by 1.5 to 2.5 fold in the dietary DHA group. In summary, feeding preformed DHA led to a marked increase in n-3 HUFA content of rat organs at the expense of n-6 HUFA and also prevented the accumulation of newly synthesized deuterated end products. This is the first study which has isolated the effects of DHA on the de novo metabolism on both the n-6 and n-3 EFA pathways.
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影响因子：
4.2
作者：
BOURRE, JM;FRANCOIS, M;DURAND, G
通讯作者：
DURAND, G
影响因子：
3.6
作者：
Kaur, Gunveen;Begg, Denovan P.;Sinclair, Andrew J.
通讯作者：
Sinclair, Andrew J.
影响因子：
168.9
作者：
DYERBERG, J;BANG, HO
通讯作者：
BANG, HO
影响因子：
6.5
作者：
Li, Y;Nara, TY;Nakamura, MT
通讯作者：
Nakamura, MT
DOI：
10.1054/plef.2002.0411
发表时间：
2002-08-01
影响因子：
3
作者：
Lin, Y;Salem, N
通讯作者：
Salem, N