Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review.
Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review.
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DOI：
10.1371/journal.pone.0248218
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Le Doare K
中科院分区：
文献类型：
--
作者：
Amini F;Auma E;Hsia Y;Bilton S;Hall T;Ramkhelawon L;Heath PT;Le Doare K
越来越多地建议在低收入和中等收入国家进行疫苗效力研究，但往往缺乏正确采集和储存婴儿血液样本的设施。干血点（DBS）对于收集用于诊断目的的婴儿血液很有用，特别是在低收入环境中，因为所需的血量很少，而且不需要冷藏。人们对它们在儿童抗体研究中的效用知之甚少。本系统综述旨在研究DBS中抗传染病抗体浓度与血清或血浆样本的相关性，这可能为疫苗临床试验提供信息。我们在MEDLINE、Embase和Cochrane图书馆检索1990年1月至2020年10月无语言限制的相关研究，使用PRISMA指南，研究DBS抗体浓度与血清或血浆样本之间的相关性，以及储存温度对DBS诊断性能的影响。我们在本系统综述中纳入了40项研究。DBS抗体浓度与血清/血浆样品有良好的合并相关性（r2 = 0.86（范围0.43 ~ 1.00））。10项研究描述了抗体在室温下保存28天后的下降，而在-20°C的最佳储存条件下，抗体可以稳定保存长达200天。只有五项关于抗菌抗体的研究。DBS中的抗体浓度与血清/血浆样本之间存在良好的相关性，这支持DBS在疫苗和血清流行病学研究中的广泛使用，但关于抗菌抗体的数据有限。DBS的正确存储是至关重要的，并且可能是长期存储的考虑因素。
Increasingly, vaccine efficacy studies are being recommended in low-and-middle-income countries (LMIC), yet often facilities are unavailable to take and store infant blood samples correctly. Dried blood spots (DBS), are useful for collecting blood from infants for diagnostic purposes, especially in low-income settings, as the amount of blood required is miniscule and no refrigeration is required. Little is known about their utility for antibody studies in children. This systematic review aims to investigate the correlation of antibody concentrations against infectious diseases in DBS in comparison to serum or plasma samples that might inform their use in vaccine clinical trials. We searched MEDLINE, Embase and the Cochrane library for relevant studies between January 1990 to October 2020 with no language restriction, using PRISMA guidelines, investigating the correlation between antibody concentrations in DBS and serum or plasma samples, and the effect of storage temperature on DBS diagnostic performance. We included 40 studies in this systematic review. The antibody concentration in DBS and serum/plasma samples reported a good pooled correlation, (r2 = 0.86 (ranged 0.43 to 1.00)). Ten studies described a decline of antibody after 28 days at room temperature compared to optimal storage at -20°C, where antibodies were stable for up to 200 days. There were only five studies of anti-bacterial antibodies. There is a good correlation between antibody concentrations in DBS and serum/plasma samples, supporting the wider use of DBS in vaccine and sero-epidemiological studies, but there is limited data on anti-bacterial antibodies. The correct storage of DBS is critical and may be a consideration for longer term storage.
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影响因子：
12.7
作者：
Colson, K. Ellicott;Potter, Alan;Mokdad, Ali H.
通讯作者：
Mokdad, Ali H.
影响因子：
3.1
作者：
Bhatia, Ramya;Stewart, June;Cuschieri, Kate
通讯作者：
Cuschieri, Kate
影响因子：
8.8
作者：
Dokubo, E. Kainne;Evans, Jennifer;Page, Kimberly
通讯作者：
Page, Kimberly
DOI：
10.1590/s0036-46652008000300004
发表时间：
2008-06-01
期刊：
Revista do Instituto de Medicina Tropical de São Paulo
影响因子：
--
作者：
Castro, Andréa Cauduro de;Borges, Luiz Gustavo dos Anjos;D'Azevedo, Pedro Alves
通讯作者：
D'Azevedo, Pedro Alves
影响因子：
4.2
作者：
Duarte, EC;Gyorkos, TW;Fontes, CJF
通讯作者：
Fontes, CJF