Sex pheromone communication in an insect parasitoid, Campoletis chlorideae Uchida.
Sex pheromone communication in an insect parasitoid, Campoletis chlorideae Uchida.
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昆虫拟寄生蜂的性信息素通讯
DOI：
10.1073/pnas.2215442119
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发表时间：
2022-12-06
影响因子：
11.1
通讯作者：
Wang, Chen-Zhu
中科院分区：
文献类型：
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作者：
Guo, Hao;Mo, Bao-Tong;Li, Guo-Cheng;Li, Zi-Lin;Huang, Ling-Qiao;Sun, Ya-Lan;Dong, Jun-Feng;Smith, Dean P.;Wang, Chen-Zhu
寄生蜂性信息素通讯的化学、行为和分子机制尚未见报道。在这里，我们已经确定了两个女性衍生的性信息素成分，十四醛（14：Ald）和2-十七酮（2-Hep）的比例为1：4.6从Campoletis chlorideae（Ichalionidae），寄生蜂，可以寄生大约30鳞翅目物种。此外，我们已经发现，CchlOR 18和CchlOR 47是两个分别调谐到14：Ald和2-Hep的受体。此外，我们已经证明，性信息素可以与雌性引诱剂（Z）-茉莉酮一起使用，以显着提高寄主幼虫的寄生率。这一发现为系统地了解拟寄生蜂的性信息素通信铺平了道路，并指出了提高其寄生能力以防治害虫的方法。性信息素是昆虫繁殖的关键。然而，性信息素的通信机制仍然是一个谜，在hyperteran寄生蜂。在这里，我们已经确定了性信息素，并阐明了性信息素通信的嗅觉基础Campoletis chlorideae（姬蜂科），一个孤立的幼虫endoparasitoid的30多个鳞翅目害虫。利用气相色谱-触角电位检测技术，我们鉴定出两种雌虫信息素成分，十四醛（14：Ald）和2-十七酮（2-Hep）（1：4.6），引起雄虫的强烈触角反应，而雌虫的反应较弱。我们观察到，男性，而不是女性被吸引到这两个单一的组件和混合物。经己烷洗涤的雌尸不能唤起雄尸，补充14：Ald和2-Hep可部分恢复雄尸的性吸引力。我们进一步表达了六个C。chlorideae雄性偏向的气味受体在果蝇T1神经元，并发现CchlOR 18和CchlOR 47选择性调谐到14：Ald和2-Hep分别。为了验证这些数据的生物学意义，我们在体内单独或一起敲除CchlOR 18和CchlOR 47，并显示C.氯离子与其各自配体的结合被消除。此外，在任何一个受体缺陷的拟寄生蜂不太可能法院和交配。最后，我们发现性信息素和（Z）-茉莉酮，一种有效的雌性引诱剂，可以协同影响处女男性和处女女性的行为，并最终提高寄生效率的C。chlorideae。我们的研究为深入了解黑腹果蝇性信息素通讯的分子机制提供了新的思路。可以操纵拟寄生虫行为以控制害虫的绿蝇科。
The chemical, behavioral, and molecular dissection of sex pheromone communication in parasitoid wasps has not been reported. Here, we have identified two female-derived sex pheromone components, tetradecanal (14:Ald) and 2-heptadecanone (2-Hep) with a ratio of 1:4.6 from Campoletis chlorideae (Ichneumonidae), a parasitoid that can parasitize approximately 30 lepidopteran species. Further, we have discovered that CchlOR18 and CchlOR47 are the two receptors tuned to 14:Ald and 2-Hep, respectively. Additionally, we have demonstrated that the sex pheromones can be applied with a female attractant, (Z)-jasmone to significantly increase the parasitism rate of host larvae. This discovery paves the way for a systematic understanding of sex pheromone communication in parasitoids and points at ways to enhance their parasitic power for pest control. Sex pheromones are pivotal for insect reproduction. However, the mechanism of sex pheromone communication remains enigmatic in hymenopteran parasitoids. Here we have identified the sex pheromone and elucidated the olfactory basis of sex pheromone communication in Campoletis chlorideae (Ichneumonidae), a solitary larval endoparasitoid of over 30 lepidopteran pests. Using coupled gas chromatography-electroantennogram detection, we identified two female-derived pheromone components, tetradecanal (14:Ald) and 2-heptadecanone (2-Hep) (1:4.6), eliciting strong antennal responses from males but weak responses from females. We observed that males but not females were attracted to both single components and the blend. The hexane-washed female cadavers failed to arouse males, and replenishing 14:Ald and 2-Hep could partially restore the sexual attraction of males. We further expressed six C. chlorideae male-biased odorant receptors in Drosophila T1 neurons and found that CchlOR18 and CchlOR47 were selectively tuned to 14:Ald and 2-Hep, respectively. To verify the biological significance of this data, we knocked down CchlOR18 and CchlOR47 individually or together in vivo and show that the attraction of C. chlorideae to their respective ligands was abolished. Moreover, the parasitoids defective in either of the receptors were less likely to court and copulate. Finally, we show that the sex pheromone and (Z)-jasmone, a potent female attractant, can synergistically affect behaviors of virgin males and virgin females and ultimately increase the parasitic efficiency of C. chlorideae. Our study provides new insights into the molecular mechanism of sex pheromone communication in C. chlorideae that may permit manipulation of parasitoid behavior for pest control.
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影响因子：
4.1
作者：
Guo，Hao;Gong，Xin-Lin;Wang，Chen-Zhu
通讯作者：
Wang，Chen-Zhu
影响因子：
2.3
作者：
Boettinger, Lea C.;Hofferberth, John;Stoekl, Johannes
通讯作者：
Stoekl, Johannes
影响因子：
2.3
作者：
Cônsoli, FL;Williams, HJ;Cooperband, MF
通讯作者：
Cooperband, MF
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1073/pnas.1718284115
发表时间：
2018-01-23
影响因子：
11.1
作者：
Choo YM;Xu P;Hwang JK;Zeng F;Tan K;Bhagavathy G;Chauhan KR;Leal WS
通讯作者：
Leal WS