Acid sensing by the Drosophila olfactory system.
Acid sensing by the Drosophila olfactory system.
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作者：
酸的气味具有明显的性质，被认为是尖锐的，辛辣的，通常是刺激性的。酸度是如何被感知并转化为适当的行为反应的，人们对此知之甚少。在这里，我们描述了一个功能分离的人口嗅觉感觉神经元的果蝇，黑腹果蝇，是高度选择性的酸度。这些嗅觉感觉神经元表达IR 64 a，IR 64 a是最近发现的嗅觉受体家族的成员之一，在活体钙离子成像显示，IR 64 a+神经元投射到触角叶中的DC 4肾小球被酸特异性激活。其中IR64a+神经元或其IR64agene的功能被破坏的果蝇在酸诱发的生理和行为反应中有缺陷，但它们对非酸性气味的反应不受影响。此外，人工刺激IR64a+神经元引起回避反应。两者合计，这些结果确定在theDrosophilaolfactory系统中的酸检测的细胞和分子底物，并支持在外围的酸度编码的标记线模式。
The odour of acids has a distinct quality that is perceived as sharp, pungent and often irritating. How acidity is sensed and translated into an appropriate behavioural response is poorly understood. Here we describe a functionally segregated population of olfactory sensory neurons in the fruitfly,Drosophila melanogaster, that are highly selective for acidity. These olfactory sensory neurons express IR64a, a member of the recently identified ionotropic receptor (IR) family of putative olfactory receptors.In vivocalcium imaging showed that IR64a+ neurons projecting to the DC4 glomerulus in the antennal lobe are specifically activated by acids. Flies in which the function of IR64a+ neurons or theIR64agene is disrupted had defects in acid-evoked physiological and behavioural responses, but their responses to non-acidic odorants remained unaffected. Furthermore, artificial stimulation of IR64a+ neurons elicited avoidance responses. Taken together, these results identify cellular and molecular substrates for acid detection in theDrosophilaolfactory system and support a labelled-line mode of acidity coding at the periphery.
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影响因子：
64.8
作者：
Yorozu, Suzuko;Wong, Allan;Fischer, Brian J.;Dankert, Heiko;Kernan, Maurice J.;Kamikouchi, Azusa;Ito, Kei;Anderson, David J.
通讯作者：
Anderson, David J.
影响因子：
64.5
作者：
Vosshall, LB;Wong, AM;Axel, R
通讯作者：
Axel, R
影响因子：
16.2
作者：
SWEENEY, ST;BROADIE, K;OKANE, CJ
通讯作者：
OKANE, CJ
影响因子：
2.3
作者：
Thom, C;Guerenstein, PG;Hildebrand, JG
通讯作者：
Hildebrand, JG
影响因子：
16.2
作者：
Ng, M;Roorda, RD;Miesenböck, G
通讯作者：
Miesenböck, G