Acute nitric oxide synthase inhibition induces greater increases in blood pressure in female versus male Wistar Kyoto rats.
Acute nitric oxide synthase inhibition induces greater increases in blood pressure in female versus male Wistar Kyoto rats.
复制标题
DOI：
10.14814/phy2.15771
复制
发表时间：
2023-08
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
--
作者：
一氧化氮（NO）通过其血管舒张和抗炎特性有助于血压（BP）调节。我们和其他人先前报道了血压正常和高血压大鼠模型中血压的性别差异，其中女性的血压低于年龄匹配的男性。由于已知雌性动物的NO生物利用度高于年龄匹配的雄性动物，因此本研究旨在检验以下假设：与雄性WKY相比，麻醉的雌性血压正常Wistar京都大鼠（WKY）对急性NOS抑制诱导的BP升高的反应更强。将12周龄雄性和雌性WKY随机输注非特异性NOS抑制剂NG-硝基-L-精氨酸甲酯（L-NAME，1 mg/kg/min）或乙烯基-L-NIO（VNIO，0.5 mg/kg/min）选择性NOS 1抑制剂60 min。在L-NAME或VNIO输注前和输注期间评估平均动脉血压、肾小球滤过率（GFR）、尿量和电解质排泄。L-NAME和VNIO显著增加了两种性别的血压;然而，与基线血压值相比，女性输注L-NAME后的血压增幅大于男性。急性输注L-NAME或VNIO 60分钟均未改变两种性别的GFR。然而，输注L-NAME和VNIO后，雄性和雌性WKY的尿量、钠、氯化物和钾排泄水平均显著增加。我们的研究结果表明，WKY中BP对急性非同种型特异性NOS抑制的反应存在性别差异，女性对L-NAME诱导的BP升高的反应比男性WKY更敏感。然而，BP反应的性别差异与急性NOS抑制时肾血流动力学反应的性别差异并不一致。
Nitric oxide (NO) contributes to blood pressure (BP) regulation via its vasodilatory and anti‐inflammatory properties. We and others previously reported sex differences in BP in normotensive and hypertensive rat models where females have lower BP than age‐matched males. As females are known to have greater NO bioavailability than age‐matched males, the current study was designed to test the hypothesis that anesthetized female normotensive Wistar Kyoto rats (WKY) are more responsive to acute NOS inhibition‐induced increases in BP compared to male WKY. Twelve‐week‐old male and female WKY were randomized to infusion of the nonspecific NOS inhibitor NG‐nitro‐L‐arginine methyl ester (L‐NAME, 1 mg/kg/min) or selective NOS1 inhibition with vinyl‐L‐NIO (VNIO, 0.5 mg/kg/min) for 60 min. Mean arterial BP, glomerular filtration rate (GFR), urine volume, and electrolyte excretion were assessed before, and during L‐NAME or VNIO infusion. L‐NAME and VNIO significantly increased BP in both sexes; however, the increase in BP with L‐NAME infusion was greater in females versus males compared to baseline BP values. Acute infusion of neither L‐NAME nor VNIO for 60 min altered GFR in either sex. However, urine volume, sodium, chloride and potassium excretion levels increased comparably in male and female WKY with L‐NAME and VNIO infusion. Our findings suggest sex differences in BP responses to acute non‐isoform‐specific NOS inhibition in WKY, with females being more responsive to L‐NAME‐induced elevations in BP relative to male WKY. However, sex differences in the BP response did not coincide with sex differences in renal hemodynamic responses to acute NOS inhibition.
登录
查看更多内容
影响因子：
5.6
作者：
Hilliard, Lucinda M.;Sampson, Amanda K.;Denton, Kate M.
通讯作者：
Denton, Kate M.
DOI：
10.1152/ajpheart.1998.275.1.h15
发表时间：
1998-07-01
影响因子：
4.8
作者：
Kähönen, M;Tolvanen, JP;Pörsti, I
通讯作者：
Pörsti, I
影响因子：
4.8
作者：
Kosaka, H;Yoneyama, H;Igarashi, J
通讯作者：
Igarashi, J
影响因子：
8.7
作者：
Ide, T;Tsutsui, H;Takeshita, A
通讯作者：
Takeshita, A
影响因子：
--
作者：
MUNGER, K;BAYLIS, C
通讯作者：
BAYLIS, C