Intense circulation of A/H5N1 and other avian influenza viruses in Cambodian live-bird markets with serological evidence of sub-clinical human infections.
Intense circulation of A/H5N1 and other avian influenza viruses in Cambodian live-bird markets with serological evidence of sub-clinical human infections.
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DOI：
10.1038/emi.2016.69
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发表时间：
2016-07-20
影响因子：
13.2
通讯作者：
Buchy, Philippe
中科院分区：
文献类型：
--
作者：
Horm, Srey Viseth;Tarantola, Arnaud;Rith, Sareth;Ly, Sowath;Gambaretti, Juliette;Duong, Veasna;Phalla, Y.;Sorn, San;Holl, Davun;Allal, Lotfi;Kalpravidh, Wantanee;Dussart, Philippe;Horwood, Paul F.;Buchy, Philippe
2013年1月至11月，在柬埔寨的四个活禽市场进行了家禽和环境样本中禽流感病毒（AIV）的监测。通过实时RT-PCR检测，在全年采集的1048份样本中，45%检出了AIV。鸭和鸡拭子的检出率分别为32%和18%，屠体清洗水样本的检出率为75%。在79%的甲型流感病毒阳性样本和35%的所有采集样本中检测到甲型流感/H5 N1病毒。对A/H5 N1病毒的全长血凝素（HA）和神经氨酸酶（NA）基因的序列分析，以及对6个代表性分离株的全基因组分析显示，2013年期间与柬埔寨人间病例相关的进化枝1.1.2重组病毒是该年唯一检测到的A/H5 N1病毒。然而，HA和NA基因的多重逆转录聚合酶链反应（RT-PCR）分析显示，至少有9种低致病性AIV（HA 1、HA 2、HA 3、HA 4、HA 6、HA 7、HA 9、HA 10和HA 11亚型）共循环。全年在125名家禽工人中进行了4次重复血清学调查。血清学检测发现，A/H5 N1和A/H9 N2抗体的总体流行率分别为4.5%和1.8%。检测到A/H5 N1和A/H9 N2的血清转换率分别为每1000人月3.7和0.9例。禽流感病毒的高峰与农历新年有关。了解禽流感在市场上传播增加的时期，应有助于采取干预措施，如清理和关闭市场，以减少人类感染和出现新的AIV的风险。
Surveillance for avian influenza viruses (AIVs) in poultry and environmental samples was conducted in four live-bird markets in Cambodia from January through November 2013. Through real-time RT-PCR testing, AIVs were detected in 45% of 1048 samples collected throughout the year. Detection rates ranged from 32% and 18% in duck and chicken swabs, respectively, to 75% in carcass wash water samples. Influenza A/H5N1 virus was detected in 79% of samples positive for influenza A virus and 35% of all samples collected. Sequence analysis of full-length haemagglutinin (HA) and neuraminidase (NA) genes from A/H5N1 viruses, and full-genome analysis of six representative isolates, revealed that the clade 1.1.2 reassortant virus associated with Cambodian human cases during 2013 was the only A/H5N1 virus detected during the year. However, multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of HA and NA genes revealed co-circulation of at least nine low pathogenic AIVs from HA1, HA2, HA3, HA4, HA6, HA7, HA9, HA10 and HA11 subtypes. Four repeated serological surveys were conducted throughout the year in a cohort of 125 poultry workers. Serological testing found an overall prevalence of 4.5% and 1.8% for antibodies to A/H5N1 and A/H9N2, respectively. Seroconversion rates of 3.7 and 0.9 cases per 1000 person-months participation were detected for A/H5N1 and A/H9N2, respectively. Peak AIV circulation was associated with the Lunar New Year festival. Knowledge of periods of increased circulation of avian influenza in markets should inform intervention measures such as market cleaning and closures to reduce risk of human infections and emergence of novel AIVs.
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影响因子：
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作者：
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通讯作者：
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