An ELISA based on recombinant spike protein S for the detection of antibodies to transmissible gastroenteritis virus of swine-like canine coronaviruses.
An ELISA based on recombinant spike protein S for the detection of antibodies to transmissible gastroenteritis virus of swine-like canine coronaviruses.
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DOI：
10.1016/j.jviromet.2009.10.015
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发表时间：
2010-03
影响因子：
3.1
通讯作者：
Buonavoglia C
中科院分区：
文献类型：
--
作者：
Elia G;Decaro N;Martella V;Lorusso E;Mari V;Maria SL;Cordioli P;Buonavoglia C
重组犬冠状病毒（Recombinant canine coronaviruses）是一种与猪传染性胃肠炎病毒（transmissible gastroenteritis virus of swine，TGEV）相似的重组病毒，其S糖蛋白的5′端片段与TGEV相似。在大肠杆菌无细胞系统中表达了TGEV样犬冠状病毒（CCoV）174/06株S蛋白的5′片段（S′）。纯化的重组多肽用于开发用于检测犬血清中TGEV样CCoV特异性抗体的ELISA试验。检测了4份TGEV样CCoV阳性犬血清、6份经典CCoV-II阳性血清和10份阴性对照血清。重组S′不被经典CCoV-II的抗体识别，因为只有实验感染TGEV样CCoV的狗的血清与重组S′多肽强烈反应，而具有经典CCoV-II抗体的狗血清不反应。由于经典CCoV-II和TGEV-like CCoV在抗原性上有一定的相关性，因此重组S′ ELISA是一种有效的检测犬TGEV-like CCoV感染的方法。
Recombinant canine coronaviruses, resembling the transmissible gastroenteritis virus of swine (TGEV) in a 5′ fragment of the S glycoprotein, have been detected recently and showed to be present in canine populations. The 5′ fragment of the S protein (S′) of a TGEV-like canine coronavirus (CCoV), strain 174/06, was expressed in an Escherichia coli cell-free system. The purified recombinant polypeptide was employed to develop an ELISA test for the detection of TGEV-like CCoV-specific antibodies in dog sera. Four canine sera positive for TGEV-like CCoV, six sera positive to classical CCoV-II strains and 10 negative control sera were examined. The recombinant S′ was not recognized by antibodies to classical CCoV-II, as only sera from dogs infected experimentally with TGEV-like CCoV reacted strongly with the recombinant S′ polypeptide whereas dog sera with antibodies to classical CCoV-II did not react. As classical CCoV-II and TEGV-like CCoVs are related antigenically, the recombinant S′ ELISA is a useful method to investigate serologically the prevalence of TGEV-like CCoVs in dogs.
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影响因子：
3.1
作者：
Decaro N;Martella V;Ricci D;Elia G;Desario C;Campolo M;Cavaliere N;Di Trani L;Tempesta M;Buonavoglia C
通讯作者：
Buonavoglia C
影响因子：
1.5
作者：
Evermann, JF;Abbott, JR;Han, S
通讯作者：
Han, S
影响因子：
5
作者：
Sanchez-Morgado, JM;Poynter, S;Morris, TH
通讯作者：
Morris, TH
影响因子：
5.4
作者：
Decaro, Nicola;Mari, Viviana;Buonavoglia, Canio
通讯作者：
Buonavoglia, Canio
影响因子：
3.1
作者：
Pratelli, A;Elia, G;Buonavoglia, C
通讯作者：
Buonavoglia, C