Intraperitoneal injection of ethanol results in drastic changes in bone metabolism not observed when ethanol is administered by oral gavage.
Intraperitoneal injection of ethanol results in drastic changes in bone metabolism not observed when ethanol is administered by oral gavage.
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DOI：
10.1111/acer.12105
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发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Turner RT
中科院分区：
文献类型：
--
作者：
Iwaniec UT;Turner RT
慢性酒精滥用与骨质疏松症风险增加有关，而轻度至中度酒精摄入与骨质疏松症风险降低有关。在流质饮食中添加酒精通常用于模拟慢性酒精滥用。间歇性饮酒（包括捆绑饮酒和轻度至中度饮酒）的模型建立方法包括1）经口灌胃给予酒精或2）经腹腔注射给予酒精。然而，尚不清楚后两种方法是否产生可比的结果。本研究的目的是确定骨骼对每日经口灌胃或ip注射给予的酒精的反应。向4月龄雌性Sprague道利大鼠给予乙醇或溶剂，每日一次，剂量为1.2 g/kg体重，持续7天。尸检后，通过组织形态计量学评价胫骨骨干（皮质骨）和胫骨近端干骺端（松质骨）的骨形成和骨结构。测定股骨远端干骺端骨基质蛋白mRNA。灌胃给予酒精对体重增加或骨测量无显著影响。相反，通过ip注射给予相同剂量的酒精导致体重减轻，胫骨骨干中骨膜骨形成的完全抑制，胫骨近端干骺端松质骨形成减少，股骨远端骨基质蛋白的mRNA水平降低。我们的研究结果引起了人们对啮齿动物ip注射乙醇作为人类间歇性暴露于酒精对骨骼影响的建模方法的关注。这一担忧是基于ip途径无法复制酒精给药的口服途径。
Chronic alcohol abuse is associated with increased risk for osteoporosis while light to moderate alcohol intake correlates with reduced osteoporosis risk. Addition of alcohol to a liquid diet is often used to model chronic alcohol abuse. Methods to model intermittent drinking (including bindge drinking and light to moderate consumption) include 1) intragastric administration of alcohol by oral gavage or 2) intraperitoneal (ip) administration of alcohol by injection. However, it is unclear whether the latter two methods produce comparable results. The purpose of this investigation was to determine the skeletal response to alcohol delivered daily by oral gavage or ip injection. Ethanol or vehicle was administered to 4-month-old female Sprague Dawley rats once daily at 1.2 g/kg body weight for 7 days. Following necropsy, bone formation and bone architecture were evaluated in tibial diaphysis (cortical bone) and proximal tibial metaphysis (cancellous bone) by histomorphometry. mRNA was measured for bone matrix proteins in distal femur metaphysis. Administration of alcohol by gavage had no significant effect on body weight gain or bone measurements. In contrast, administration of the same dose of alcohol by ip injection resulted in reduced body weight, total suppression of periosteal bone formation in tibial diaphysis, decreased cancellous bone formation in proximal tibial metaphysis, and decreased mRNA levels for bone matrix proteins in distal femur. Our findings raise concerns regarding the use of ip injection of ethanol in rodents as a method for modeling the skeletal effects of intermittent exposure to alcohol in humans. This concern is based on a failure of the ip route to replicate the oral route of alcohol administration.
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DOI：
10.1097/00000374-200111000-00014
发表时间：
2001-11-01
影响因子：
3.2
作者：
Knapp, DJ;Braun, CJ;Breese, GR
通讯作者：
Breese, GR
DOI：
10.1097/gme.0b013e31824ac071
发表时间：
2012-09-01
影响因子：
2.7
作者：
Marrone, Jill A.;Maddalozzo, Gianni F.;Iwaniec, Urszula T.
通讯作者：
Iwaniec, Urszula T.
影响因子：
4
作者：
Maddalozzo, G. F.;Turner, R. T.;Iwaniec, U. T.
通讯作者：
Iwaniec, U. T.
影响因子：
3.1
作者：
Morales-González, JA;Gutiérrez-Salinas, J;Hernández-Muñoz, R
通讯作者：
Hernández-Muñoz, R
影响因子：
6.2
作者：
Devlin, Maureen J.;Cloutier, Alison M.;Thomas, Nishina A.;Panus, David A.;Lotinun, Sutada;Pinz, Ilka;Baron, Roland;Rosen, Clifford J.;Bouxsein, Mary L.
通讯作者：
Bouxsein, Mary L.