Human ribosomal protein S13 promotes gastric cancer growth through down-regulating p27(Kip1).
Human ribosomal protein S13 promotes gastric cancer growth through down-regulating p27(Kip1).
复制标题
人核糖体蛋白S13通过下调p27Kip1促进胃癌生长
DOI：
10.1111/j.1582-4934.2009.00969.x
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发表时间：
2011-02
影响因子：
5.3
通讯作者：
Fan D
中科院分区：
文献类型：
--
作者：
Guo X;Shi Y;Gou Y;Li J;Han S;Zhang Y;Huo J;Ning X;Sun L;Chen Y;Sun S;Fan D
我们的前期工作发现，人核糖体蛋白S13（RPS13）在胃癌多药耐药细胞中表达上调，RPS13过表达可保护胃癌细胞免受药物诱导的凋亡。本研究旨在探讨RPS13在胃癌发生发展中的作用。采用免疫组化染色和Western印迹分析方法检测RPS 13在胃癌组织和正常胃黏膜中的表达。结果发现RPS13在胃癌组织中的表达水平高于正常胃粘膜组织。然后，RPS13在胃癌细胞中基因过表达或通过RNA干扰敲低。研究表明，RPS13基因的上调可促进胃癌细胞的生长，增强其体外集落形成能力和软琼脂集落形成能力，并促进其体内成瘤能力。同时，在胃癌细胞中RPS13的下调导致完全相反的效果。此外，RPS13的过表达可促进胃癌细胞的G1期向S期的转变，而RPS13的敲低则导致胃癌细胞的G1期阻滞。RPS13下调p27kip1表达和CDK2激酶活性，但不影响cyclin D、cyclin E、CDK2、CDK4和p16INK4A的表达。综上所述，RPS13至少可以通过抑制p27kip1的表达来促进胃癌细胞的生长和细胞周期进程。
Our previous works revealed that human ribosomal protein S13 (RPS13) was up-regulated in multidrug-resistant gastric cancer cells and overexpression of RPS13 could protect gastric cancer cells from drug-induced apoptosis. The present study was designed to explore the role of RPS13 in tumorigenesis and development of gastric cancer. The expression of RPS13 in gastric cancer tissues and normal gastric mucosa was evaluated by immunohistochemical staining and Western blot analysis. It was found RPS13 was expressed at a higher level in gastric cancer tissues than that in normal gastric mucosa. RPS13 was then genetically overexpressed in gastric cancer cells or knocked down by RNA interference. It was demonstrated that up-regulation of RPS13 accelerated the growth, enhanced in vitro colony forming and soft agar cologenic ability and promoted in vivo tumour formation potential of gastric cancer cells. Meanwhile, down-regulation of RPS13 in gastric cancer cells resulted in complete opposite effects. Moreover, overexpression of RPS13 could promote G1 to S phase transition whereas knocking down of RPS13 led to G1 arrest of gastric cancer cells. It was further demonstrated that RPS13 down-regulated p27kip1 expression and CDK2 kinase activity but did not change the expression of cyclin D, cyclin E, CDK2, CDK4 and p16INK4A. Taken together, these data indicate that RPS13 could promote the growth and cell cycle progression of gastric cancer cells at least through inhibiting p27kip1 expression.
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影响因子：
4.8
作者：
Liang, Jie;Pan, Yanglin;Fan, Daiming
通讯作者：
Fan, Daiming
DOI：
10.1016/j.bbrc.2005.10.064
发表时间：
2005-12-16
影响因子：
3.1
作者：
Kim, MJ;Yoo, YA;Yoo, YD
通讯作者：
Yoo, YD
影响因子：
6.4
作者：
DENIS, MG;CHADENEAU, C;LUSTENBERGER, P
通讯作者：
LUSTENBERGER, P
影响因子：
4
作者：
Naora, H;Naora, H
通讯作者：
Naora, H
影响因子：
3.5
作者：
Jang, CY;Lee, JY;Kim, J
通讯作者：
Kim, J