A widespread self-cleaving ribozyme class is revealed by bioinformatics.
A widespread self-cleaving ribozyme class is revealed by bioinformatics.
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DOI：
10.1038/nchembio.1386
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发表时间：
2014-01
影响因子：
14.8
通讯作者：
Breaker, Ronald R.
中科院分区：
文献类型：
--
作者：
Roth, Adam;Weinberg, Zasha;Chen, Andy G. Y.;Kim, Peter B.;Ames, Tyler D.;Breaker, Ronald R.
核酶是非编码 RNA，可促进化学转化，其速率增强接近蛋白酶。尽管核酶在 RNA 世界时代很可能非常丰富，但目前已知的生物体中只存在十类核酶。我们报告了一种额外的自裂解核酶类的发现和分析，称为twister，它存在于许多细菌和真核生物中。鉴定出近 2700 个twister核酶，它们符合小而复杂的二级结构共识，具有由内部环和末端环连接的三个茎。两个假结提供了对于催化活性至关重要的三级结构接触。扭转核酶基序提供了天然 RNA 催化剂的另一个例子，并引起人们对此类和其他广泛分布的自切割 RNA 类别的潜在不同生物学作用的关注。
Ribozymes are noncoding RNAs that promote chemical transformations with rate enhancements approaching those of protein enzymes. Although ribozymes are likely to have been abundant during the RNA world era, only ten classes are known to exist among contemporary organisms. We report the discovery and analysis of an additional self-cleaving ribozyme class, called twister, which is present in many species of bacteria and eukarya. Nearly 2700 twister ribozymes were identified that conform to a secondary structure consensus that is small yet complex, with three stems conjoined by internal and terminal loops. Two pseudoknots provide tertiary structure contacts that are critical for catalytic activity. The twister ribozyme motif provides another example of a natural RNA catalyst and calls attention to the potentially varied biological roles of this and other classes of widely distributed self-cleaving RNAs.
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影响因子：
3.9
作者：
Cech, TR
通讯作者：
Cech, TR
影响因子：
7.2
作者：
Lambowitz, Alan M.;Zimmerly, Steven
通讯作者：
Zimmerly, Steven
影响因子：
4.5
作者：
Breaker, RR;Emilsson, GM;Sudarsan, N
通讯作者：
Sudarsan, N
DOI：
10.1073/pnas.86.18.7054
发表时间：
1989-09-01
影响因子：
11.1
作者：
BENNER, SA;ELLINGTON, AD;TAUER, A
通讯作者：
TAUER, A
影响因子：
64.8
作者：
BUZAYAN, JM;GERLACH, WL;BRUENING, G
通讯作者：
BRUENING, G