Control of vein-forming, striped gene expression by auxin signaling.
Control of vein-forming, striped gene expression by auxin signaling.
复制标题
生长素信号调控血管形成、条纹基因表达。
DOI：
10.1186/s12915-021-01143-9
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发表时间：
2021-09-24
期刊：
影响因子：
5.4
通讯作者：
Scarpella E
中科院分区：
文献类型：
--
作者：
Krishna A;Gardiner J;Donner TJ;Scarpella E
条纹结构域中基因表达的激活是生物模式的关键组成部分，从植物叶子中静脉的递归形成到我们体内肋骨和椎骨的递归形成。在动物中，通过广泛表达的转录因子对其靶基因的差异亲和力以及这些靶基因之间的组合相互作用，在条纹结构域中激活基因表达。在植物中，条纹结构域中的基因表达如何被激活尚不清楚。我们针对广泛表达的 MONOPTEROS (MP) 转录因子及其靶基因拟南芥同源盒因子 8 (ATHB8) 解决了这个问题。我们发现 ATHB8 促进静脉形成，并且这种静脉形成功能取决于 ATHB8 表达水平和 ATHB8 表达域宽度。我们进一步发现，条纹结构域中的 ATHB8 表达是通过以下组合激活的：(1) 通过将峰值水平的 MP 结合到 ATHB8 启动子中的低亲和力 MP 结合位点来激活 ATHB8 表达，以及 (2) AUXIN/INDOLE-3-ACETIC-ACID-INDUCIBLE 家族的 MP 靶基因抑制 ATHB8 表达。我们的研究结果表明，尽管动植物的多细胞性是独立进化的，但共同的调控逻辑控制着植物和动物条纹结构域中基因表达的激活。在线版本包含可在 10.1186/s12915-021-01143-9 获取的补充材料。
Activation of gene expression in striped domains is a key building block of biological patterning, from the recursive formation of veins in plant leaves to that of ribs and vertebrae in our bodies. In animals, gene expression is activated in striped domains by the differential affinity of broadly expressed transcription factors for their target genes and the combinatorial interaction between such target genes. In plants, how gene expression is activated in striped domains is instead unknown. We address this question for the broadly expressed MONOPTEROS (MP) transcription factor and its target gene ARABIDOPSIS THALIANA HOMEOBOX FACTOR8 (ATHB8). We find that ATHB8 promotes vein formation and that such vein-forming function depends on both levels of ATHB8 expression and width of ATHB8 expression domains. We further find that ATHB8 expression is activated in striped domains by a combination of (1) activation of ATHB8 expression through binding of peak levels of MP to a low-affinity MP-binding site in the ATHB8 promoter and (2) repression of ATHB8 expression by MP target genes of the AUXIN/INDOLE-3-ACETIC-ACID-INDUCIBLE family. Our findings suggest that a common regulatory logic controls activation of gene expression in striped domains in both plants and animals despite the independent evolution of their multicellularity. The online version contains supplementary material available at 10.1186/s12915-021-01143-9.
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影响因子：
9.2
作者：
Heisler, MG;Ohno, C;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
生长素通过单翅目起作用，调节植物细胞极性和模式植物。
影响因子：
9.2
作者：
Bhatia, Neha;Bozorg, Behruz;Larsson, Andre;Ohno, Carolyn;Jonsson, Henrik;Heisler, Marcus G.
通讯作者：
Heisler, Marcus G.
影响因子：
2.5
作者：
Gardiner, Jason;Donner, Tyler J.;Scarpella, Enrico
通讯作者：
Scarpella, Enrico
影响因子：
4.6
作者：
Hardtke, CS;Ckurshumova, W;Berleth, T
通讯作者：
Berleth, T
影响因子：
7.4
作者：
Baima, S;Possenti, M;Morelli, G
通讯作者：
Morelli, G