Fox on the run--molecular surveillance of fox blood and tissue for the occurrence of tick-borne pathogens in Austria.

Fox on the run--molecular surveillance of fox blood and tissue for the occurrence of tick-borne pathogens in Austria.
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DOI:
10.1186/s13071-014-0521-7
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发表时间:
2014-11-21
影响因子:
3.2
通讯作者:
Kübber-Heiss A
Kübber-Heiss A
中科院分区:
医学2区
文献类型:
--
作者:
Duscher GG;Fuehrer HP;Kübber-Heiss A

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赤狐(Vulpes vulpes)是一种分布广泛的物种,携带许多与人类和宠物相关的病原体。事实上,无形体属,犬埃立克体和立克次体属。在细菌和犬肝原虫以及寄生虫中的巴贝虫属一直是几项研究的重点。在奥地利东北部一天内射杀的36只狐狸中,50%的狐狸被发现有类似巴贝氏小孢子虫的病原体,而H。在58.3%的样本中检测到犬。脾脏对H.犬,而B.在血液中更常见的是小孢子虫样寄生虫。使用PCR和对血液和脾脏样本进行测序,无法在任何病例中证实细菌,以证明此类蜱传病原体的发生。B的出现。microti-like和H.犬寄生虫引起了许多问题,因为这些感染从来没有在狗身上发现过。此外,在H.犬蜱的主要病媒蜱Rhipicephalus sanguineus在采样区不存在,这为进一步假设传播留下了空间,例如垂直传播、通过摄食猎物动物或其他病媒蜱传播。需要进一步的研究来评估宠物在这方面的风险。PCR对不同组织的结果不同,表明同时使用脾脏和血液可获得完整的结果。
The red fox (Vulpes vulpes) is a widespread species, harbouring many pathogens relevant for humans and pets. Indeed, Anaplasma spp., Ehrlichia canis and Rickettsia spp. among the bacteria and Hepatozoon canis as well as Babesia sp. among the parasites have been the focus of several studies. In a cohort of 36 foxes shot on one day in the north-eastern part of Austria, Babesia microti-like pathogens were found in 50%, while H. canis was detected in 58.3% of the samples. The spleen was more useful for detection of H. canis, whereas B. microti-like parasites were more frequently found in the blood. Bacteria could not be confirmed in any of the cases to demonstrate the occurrence of such tick-borne pathogens using PCR and sequencing on blood and spleen samples. The occurrence of B. microti-like and H. canis parasites raised many questions, because these infections have never been found autochthonously in dogs. Furthermore in the case of H. canis the main vector tick, Rhipicephalus sanguineus, is absent in the sampling area, leaving space for further hypotheses for transmission such as vertical transmission, transmission via ingestion of prey animals or other vector ticks. Further studies are needed to evaluate the risks for pets in this area. PCRs delivered differing results with the different tissues, suggesting the use of both spleen and blood to obtain an integral result.
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