Bacterial population dynamics in a laboratory activated sludge reactor monitored by pyrosequencing of 16S rRNA.
Bacterial population dynamics in a laboratory activated sludge reactor monitored by pyrosequencing of 16S rRNA.
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DOI：
10.1264/jsme2.me12115
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发表时间：
2013
影响因子：
2.2
通讯作者：
Mino T
中科院分区：
文献类型：
--
作者：
Satoh H;Oshima K;Suda W;Ranasinghe P;Li N;Gunawardana EG;Hattori M;Mino T
通过16S rRNA的焦磷酸测序，对实验室活性污泥反应器中的微生物种群进行了245d、75个时间点的监测。采用人工合成废水作为进水，整个实验过程中反应器运行在相同的条件下。观察了不同细菌操作分类单位(OTU)的行为。多个OTUS表现为周期性传播和退缩。其中一个OTU显示出急剧衰退，这表明OTU中的细胞被选择性地杀死。对不同系统发育的假丝酵母菌的行为也进行了可视化研究。研究表明，条形码对焦磷酸测序是研究活性污泥中细菌种群动态的一种非常有效的工具。
The microbial population in a laboratory activated sludge reactor was monitored for 245 d at 75 time points by pyrosequencing of 16S rRNA. Synthetic wastewater was used as the influent, and the reactor was operated under the same conditions throughout the experiment. The behaviors of different bacterial operational taxonomic units (OTUs) were observed. Multiple OTUs showed periodic propagation and recession. One of the OTUs showed sharp recession, which suggests that cells in the OTU were selectively killed. The behaviors of different phylogenetic lineages of Candidatus ‘Accumulibacter phosphatis’ were also visualized. It was clearly demonstrated that pyrosequencing with barcoded primers is a very effective tool to clarify the dynamics of the bacterial population in activated sludge.
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影响因子：
4.2
作者：
Barr, Jeremy J.;Slater, Frances R.;Bond, Philip L.
通讯作者：
Bond, Philip L.
影响因子：
4.4
作者：
Kong, YH;Nielsen, JL;Nielsen, PH
通讯作者：
Nielsen, PH
影响因子：
4.4
作者：
Gentile, M. E.;Jessup, C. M.;Criddle, C. S.
通讯作者：
Criddle, C. S.
影响因子：
3.7
作者：
Andersson, Anders F.;Lindberg, Mathilda;Jakobsson, Hedvig;Backhed, Fredrik;Nyren, Pal;Engstrand, Lars
通讯作者：
Engstrand, Lars
影响因子：
3.6
作者：
Otawa, Kenichi;Lee, Sang Hyon;Mino, Takashi
通讯作者：
Mino, Takashi