Metagenomics Shines Light on the Evolution of "Sunscreen" Pigment Metabolism in the Teloschistales (Lichen-Forming Ascomycota).
Metagenomics Shines Light on the Evolution of "Sunscreen" Pigment Metabolism in the Teloschistales (Lichen-Forming Ascomycota).
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DOI：
10.1093/gbe/evad002
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发表时间：
2023-02-03
影响因子：
3.3
通讯作者：
Gaya, Ester
中科院分区：
文献类型：
--
作者：
Llewellyn, Theo;Nowell, Reuben W.;Aptroot, Andre;Temina, Marina;Prescott, Thomas A. K.;Barraclough, Timothy G.;Gaya, Ester
关键词：
真菌产生大量的次级代谢产物，这些次级代谢产物塑造了它们与其他生物和环境的相互作用。因此，表征支持代谢物合成的基因是理解真菌进化和适应的关键。地衣化真菌几乎占子囊菌门多样性的三分之一，并拥有令人印象深刻的次级代谢产物库。然而，大多数地衣生物合成基因尚未与其代谢产物联系起来。在这里，我们使用宏基因组测序，调查与生产蒽醌，紫外线保护剂的次级代谢产物存在于各种真菌，但特别是丰富的地衣，端裂目（类蜡蚧菌，子囊菌门）的基因家族。我们成功地组装了24个新的，高质量的地衣化真菌基因组从头开始，并将它们与公开可用的来自具有不同次级化学的分类群的蜡蚧菌属基因组相结合，以产生全基因组树。次级代谢物生物合成基因簇（BGC）的分析表明，虽然地衣BGC是众多的，高度不同的，核心酶基因通常是保守的类群。这表明代谢物多样化通过用新的辅助基因重新改组现有的酶基因而不是BGC获得/损失或从头基因进化发生。我们确定了推定的蒽醌BGC在我们的地衣数据集，出现同源的蒽醌簇从非地衣真菌，这表明这些基因存在于亚门盘菌亚门的共同祖先。最后，我们确定了独特的转运蛋白基因的Teloschistales蒽醌BGC，可以解释为什么这些代谢物是如此丰富，无处不在的这些地衣。我们的研究结果支持宏基因组学的重要性，了解次生代谢的非模式真菌，如地衣。
Fungi produce a vast number of secondary metabolites that shape their interactions with other organisms and the environment. Characterizing the genes underpinning metabolite synthesis is therefore key to understanding fungal evolution and adaptation. Lichenized fungi represent almost one-third of Ascomycota diversity and boast impressive secondary metabolites repertoires. However, most lichen biosynthetic genes have not been linked to their metabolite products. Here we used metagenomic sequencing to survey gene families associated with production of anthraquinones, UV-protectant secondary metabolites present in various fungi, but especially abundant in a diverse order of lichens, the Teloschistales (class Lecanoromycetes, phylum Ascomycota). We successfully assembled 24 new, high-quality lichenized-fungal genomes de novo and combined them with publicly available Lecanoromycetes genomes from taxa with diverse secondary chemistry to produce a whole-genome tree. Secondary metabolite biosynthetic gene cluster (BGC) analysis showed that whilst lichen BGCs are numerous and highly dissimilar, core enzyme genes are generally conserved across taxa. This suggests metabolite diversification occurs via re-shuffling existing enzyme genes with novel accessory genes rather than BGC gains/losses or de novo gene evolution. We identified putative anthraquinone BGCs in our lichen dataset that appear homologous to anthraquinone clusters from non-lichenized fungi, suggesting these genes were present in the common ancestor of the subphylum Pezizomycotina. Finally, we identified unique transporter genes in Teloschistales anthraquinone BGCs that may explain why these metabolites are so abundant and ubiquitous in these lichens. Our results support the importance of metagenomics for understanding the secondary metabolism of non-model fungi such as lichens.
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影响因子：
14.9
作者：
Blum M;Chang HY;Chuguransky S;Grego T;Kandasaamy S;Mitchell A;Nuka G;Paysan-Lafosse T;Qureshi M;Raj S;Richardson L;Salazar GA;Williams L;Bork P;Bridge A;Gough J;Haft DH;Letunic I;Marchler-Bauer A;Mi H;Natale DA;Necci M;Orengo CA;Pandurangan AP;Rivoire C;Sigrist CJA;Sillitoe I;Thanki N;Thomas PD;Tosatto SCE;Wu CH;Bateman A;Finn RD
通讯作者：
Finn RD
影响因子：
4.4
作者：
Chiang, Yi-Ming;Szewczyk, Edyta;Oakley, Berl R.
通讯作者：
Oakley, Berl R.
影响因子：
5.1
作者：
Bertrand, Robert L.;Abdel-Hameed, Mona;Sorensen, John L.
通讯作者：
Sorensen, John L.
影响因子：
10.7
作者：
Hoang DT;Chernomor O;von Haeseler A;Minh BQ;Vinh LS
通讯作者：
Vinh LS
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T