Recruitment of bone marrow-derived cells to periodontal tissue defects.
Recruitment of bone marrow-derived cells to periodontal tissue defects.
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DOI：
10.3389/fcell.2014.00019
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发表时间：
2014
影响因子：
5.5
通讯作者：
Morita I
中科院分区：
文献类型：
--
作者：
Kimura Y;Komaki M;Iwasaki K;Sata M;Izumi Y;Morita I
骨髓来源的细胞(BMC)被认为是成人间充质干细胞(MSCs)的主要来源，并被认为在牙周组织再生中有效。然而，内源性BMCs是否参与牙周组织修复过程尚不确定。因此，我们在骨髓嵌合小鼠的下颌第一磨牙颊槽骨中建立了牙周组织缺损，并用免疫组织化学方法检测了基质细胞衍生因子-1(SDF-1)的表达和骨髓细胞的动员。结果发现，损伤后1周，缺损区周围SDF-1表达增强，骨髓细胞被动员到缺损区，GFP+/CD45+很少见。流式细胞仪分析显示，创伤后骨髓中血小板衍生生长因子受体α+/Sca-1+(PαS)细胞数量减少。综上所述，这些结果表明骨髓基质细胞被动员到牙周组织的缺损处。骨髓基质细胞的募集，包括一部分间充质干细胞，可能成为牙周治疗的新靶点。
Bone marrow-derived cells (BMCs) are considered to be a major source of mesenchymal stem cells (MSCs) in adults and are known to be effective in periodontal tissue regeneration. However, whether endogenous BMCs are involved in periodontal tissue repair process is uncertain. We therefore created periodontal tissue defects in the buccal alveolar bone of mandibular first molars in bone marrow chimeric mice, and immunohistochemically examined the expression of stromal cell derived factor-1 (SDF-1) and the mobilization of BMCs. We found that SDF-1 expression was increased around the defects at as early as 1 week after injury and that BMCs were mobilized to the defects, while GFP+/CD45+ were rarely observed. Fluorescence-activated cell sorting (FACS) analysis demonstrated that the number of platelet-derived growth factor receptor (pdgfr) α+/Sca-1+ (PαS) cells in the bone marrow decreased after injury. Taken together, these results suggest that BMCs are mobilized to the periodontal tissue defects. Recruitment of BMCs, including a subset of MSCs could be a new target of periodontal treatment.
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小鼠骨髓中多能间充质干细胞的前瞻性鉴定、分离和系统移植。
DOI：
10.1084/jem.20091046
发表时间：
2009-10-26
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Morikawa S;Mabuchi Y;Kubota Y;Nagai Y;Niibe K;Hiratsu E;Suzuki S;Miyauchi-Hara C;Nagoshi N;Sunabori T;Shimmura S;Miyawaki A;Nakagawa T;Suda T;Okano H;Matsuzaki Y
通讯作者：
Matsuzaki Y
影响因子：
5
作者：
Fukuda, Daiju;Enomoto, Soichiro;Sata, Masataka
通讯作者：
Sata, Masataka
影响因子：
6
作者：
Jones, Gemma N.;Moschidou, Dafni;Guillot, Pascale V.
通讯作者：
Guillot, Pascale V.
影响因子：
0.9
作者：
Mansilla, E.;Marin, G. H.;Soratti, C.
通讯作者：
Soratti, C.
影响因子：
4.3
作者：
Kawaguchi, H;Hirachi, A;Kurihara, H
通讯作者：
Kurihara, H