Spatial Assessment of Heterogeneous Tissue Natural Frequency Using Micro-Force Optical Coherence Elastography.
Spatial Assessment of Heterogeneous Tissue Natural Frequency Using Micro-Force Optical Coherence Elastography.
复制标题
使用微力光学相干弹性成像对异质组织固有频率进行空间评估
DOI：
10.3389/fbioe.2022.851094
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发表时间：
2022
影响因子：
5.7
通讯作者：
Twa MD
中科院分区：
文献类型：
--
作者：
Lan G;Shi Q;Wang Y;Ma G;Cai J;Feng J;Huang Y;Gu B;An L;Xu J;Qin J;Twa MD
角膜组织固有频率的分析最近被提出作为角膜生物力学的生物标志物，并且已经使用基于高分辨率光学相干断层扫描（OCT）的弹性成像（OCE）进行。然而，它仍然是未知的固有频率分析是否可以解决组织结构的局部变化。我们测量了异质样本，以评估自然频率分布和区域结构变化之间的对应关系。通过微升空气脉冲（60-85 Pa，3 ms）逐点诱导亚微米样品振荡，并使用具有0.44 nm相位检测灵敏度的1，300 nm谱域共光路OCT系统在每个点处相应地检测亚微米样品振荡。通过快速傅里叶变换分析了振动频率特性，并采用单自由度模型表征了固有频率。各测点的振荡特征表现为多频率分量的复杂频率响应，与整体结构特征相对应;各测点的频率幅值变化反映了局部样本特征。通过固有频率可清楚区分琼脂基质（355.6 ± 0.8 Hz和361.3 ± 5.5 Hz）中包埋的硅胶块（255.1 ± 11.0 Hz和249.0 ± 4.6 Hz）。在牛小腿样本中，中央脂肪和结缔组织的自然频率（91.7 ± 58.2 Hz）低于肌肉组织（左侧：252.6 ± 52.3 Hz;右侧：161.5 ± 35.8 Hz）。作为第一步，我们已经证明了固有频率OCE方法来表征非均匀样本的全局和局部特征的可能性。该方法可以提供关于角膜特性的额外信息，补充当前的临床生物力学评估，并且可以成为眼科疾病的临床检测和医学或手术治疗结果的评估的有用工具。
Analysis of corneal tissue natural frequency was recently proposed as a biomarker for corneal biomechanics and has been performed using high-resolution optical coherence tomography (OCT)-based elastography (OCE). However, it remains unknown whether natural frequency analysis can resolve local variations in tissue structure. We measured heterogeneous samples to evaluate the correspondence between natural frequency distributions and regional structural variations. Sub-micrometer sample oscillations were induced point-wise by microliter air pulses (60–85 Pa, 3 ms) and detected correspondingly at each point using a 1,300 nm spectral domain common path OCT system with 0.44 nm phase detection sensitivity. The resulting oscillation frequency features were analyzed via fast Fourier transform and natural frequency was characterized using a single degree of freedom (SDOF) model. Oscillation features at each measurement point showed a complex frequency response with multiple frequency components that corresponded with global structural features; while the variation of frequency magnitude at each location reflected the local sample features. Silicone blocks (255.1 ± 11.0 Hz and 249.0 ± 4.6 Hz) embedded in an agar base (355.6 ± 0.8 Hz and 361.3 ± 5.5 Hz) were clearly distinguishable by natural frequency. In a beef shank sample, central fat and connective tissues had lower natural frequencies (91.7 ± 58.2 Hz) than muscle tissue (left side: 252.6 ± 52.3 Hz; right side: 161.5 ± 35.8 Hz). As a first step, we have shown the possibility of natural frequency OCE methods to characterize global and local features of heterogeneous samples. This method can provide additional information on corneal properties, complementary to current clinical biomechanical assessments, and could become a useful tool for clinical detection of ocular disease and evaluation of medical or surgical treatment outcomes.
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DOI：
10.1007/s00417-011-1897-0
发表时间：
2012-04-01
影响因子：
2.7
作者：
Gkika, Maria;Labiris, Georgios;Kozobolis, Vassilios
通讯作者：
Kozobolis, Vassilios
影响因子：
3.4
作者：
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通讯作者：
Twa, Michael D.
DOI：
10.1016/j.jmbbm.2016.11.004
发表时间：
2017-02
影响因子：
3.9
作者：
Han Z;Li J;Singh M;Wu C;Liu CH;Raghunathan R;Aglyamov SR;Vantipalli S;Twa MD;Larin KV
通讯作者：
Larin KV
影响因子：
2.4
作者：
Lan G;Aglyamov S;Larin KV;Twa MD
通讯作者：
Twa MD
影响因子：
1.4
作者：
Hon, Ying;Lam, Andrew K. C.
通讯作者：
Lam, Andrew K. C.