Differential localization patterns of septins during growth of the human fungal pathogen Aspergillus fumigatus reveal novel functions.
Differential localization patterns of septins during growth of the human fungal pathogen Aspergillus fumigatus reveal novel functions.
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DOI：
10.1016/j.bbrc.2011.01.017
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发表时间：
2011-02-11
影响因子：
3.1
通讯作者：
Steinbach WJ
中科院分区：
文献类型：
--
作者：
Juvvadi PR;Fortwendel JR;Rogg LE;Steinbach WJ
Septins是一个保守的GTP酶家族，是一种与细胞膜和细胞骨架结合的异聚体蛋白，在细胞分裂和形态发生中起重要作用。它们在真菌细胞壁几丁质沉积、分隔、胞质分裂和孢子形成中的作用已经得到很好的确立，并且它们最近被认为与白色念珠菌的组织侵袭和毒力有关。在人类致病真菌烟曲霉中从未研究过隔膜素，烟曲霉是免疫功能低下患者死亡的主要原因。在这里，我们定位所有5 septins（AspA-E）从烟曲霉的第一次，并显示，每5 septins表现出不同的分布模式。有趣的是，AspE，这是唯一的丝状真菌，和AspD，属于CDC 10类的septins，本地化显着的管状结构，这是依赖于肌动蛋白和微管网络。AspD和AspE在丝状真菌中的定位未见报道。这些结果表明，A.烟曲霉在形态建成和致病性方面可能具有独特的功能。
Septins, a conserved family of GTPases, are heteropolymeric filament-forming proteins that associate with the cell membrane and cytoskeleton and serve essential functions in cell division and morphogenesis. Their roles in fungal cell wall chitin deposition, septation, cytokinesis, and sporulation have been well established and they have recently been implicated in tissue invasion and virulence in Candida albicans. Septins have never been investigated in the human pathogenic fungus, Aspergillus fumigatus, which is a leading cause of death in immunocompromised patients. Here we localize all the 5 septins (AspA-E) from Aspergillus fumigatus for the first time, and show that each of the 5 septins exhibit varied patterns of distribution. Interestingly AspE, which is unique to filamentous fungi, and AspD, belonging to the CDC10 class of septins, localized prominently to tubular structures which were dependent on actin and microtubule networks. Localization of AspD and AspE has never been reported in filamentous fungi. Taken together these results suggest that septins in A. fumigatus might have unique functions in morphogenesis and pathogenicity.
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