Two-photon Luminescence Imaging of Bacillus Spores Using Peptide-functionalized Gold Nanorods.
Two-photon Luminescence Imaging of Bacillus Spores Using Peptide-functionalized Gold Nanorods.
复制标题
DOI：
10.1007/s12274-008-8047-y
复制
发表时间：
2008-12-01
期刊：
影响因子：
9.9
通讯作者：
Wei, Alexander
中科院分区：
文献类型：
--
作者：
He, Wei;Henne, Walter A.;Wei, Qingshan;Zhao, Yan;Doorneweerd, Derek D.;Cheng, Ji-Xin;Low, Philip S.;Wei, Alexander
枯草芽孢杆菌孢子（炭疽芽孢杆菌的一种模拟物）已被双光子发光（TPL）显微镜成像，使用金纳米棒（gnr）功能化与半胱氨酸末端归甲肽。使用氨基酸序列混乱的肽进行对照实验，证实了GNR靶向对孢子表面具有高度选择性。TPL的高灵敏度与肽标签的高亲和力相结合，使孢子能够在飞摩尔浓度下使用gnr进行高保真度检测。研究还确定，即使在远离其纵向等离子体共振（LPR）的近红外（NIR）波长照射下，GNR也能产生显著的TPL输出，这使得选择GNR宽高比或激发波长用于TPL成像具有相当大的灵活性。
Bacillus subtilis spores (a simulant of Bacillus anthracis) have been imaged by two-photon luminescence (TPL) microscopy, using gold nanorods (GNRs) functionalized with a cysteine-terminated homing peptide. Control experiments using a peptide with a scrambled amino acid sequence confirmed that the GNR targeting was highly selective for the spore surfaces. The high sensitivity of TPL combined with the high affinity of the peptide labels enables spores to be detected with high fidelity using GNRs at femtomolar concentrations. It was also determined that GNRs are capable of significant TPL output even when irradiated at near infrared (NIR) wavelengths far from their longitudinal plasmon resonance (LPR), permitting considerable flexibility in the choice of GNR aspect ratio or excitation wavelength for TPL imaging.
登录
查看更多内容
影响因子：
15
作者：
Imura, K;Nagahara, T;Okamoto, H
通讯作者：
Okamoto, H
影响因子：
10.8
作者：
Durr, Nicholas J.;Larson, Timothy;Ben-Yakar, Adela
通讯作者：
Ben-Yakar, Adela
影响因子：
3.9
作者：
Huff, Terry B.;Hansen, Matthew N.;Wei, Alexander
通讯作者：
Wei, Alexander
影响因子：
4
作者：
Kim, Kang;Huang, Sheng-Wen;Kaplan, Mariana J.
通讯作者：
Kaplan, Mariana J.
影响因子：
82.9
作者：
Brown, EB;Campbell, RB;Jain, RK
通讯作者：
Jain, RK