Phylogenomics of the oxidative phosphorylation in fungi reveals extensive gene duplication followed by functional divergence.
Phylogenomics of the oxidative phosphorylation in fungi reveals extensive gene duplication followed by functional divergence.
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DOI：
10.1186/1471-2148-9-295
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发表时间：
2009-12-21
影响因子：
3.4
通讯作者：
Gabaldón T
中科院分区：
文献类型：
--
作者：
Marcet-Houben M;Marceddu G;Gabaldón T
氧化磷酸化是细胞能量代谢的核心。由于适应不同的生活方式和环境，真菌物种在进化过程中形成了它们的呼吸途径。为了确定呼吸途径进化背后的主要机制，我们对60种真菌基因组中的氧化磷酸化组分进行了基因组学调查。除了澄清呼吸蛋白之间的直系和旁系关系，我们的研究结果揭示了三个平行的损失，整个复杂的I，其中两个是耦合到复制的替代酶。呼吸蛋白的重复很常见，影响了76%的蛋白质家族。我们检测到几个例子的旁系同源基因编码的呼吸复合物的亚基已被招募到其他多蛋白复合物内部和外部的cupon，强调进化修补的作用。在真菌呼吸作用的进化中，基因丢失和基因复制以及随后的功能分歧过程一直很猖獗。总的来说，呼吸途径的核心蛋白在大多数谱系中是保守的，由于适应厌氧生活方式，微孢子虫、裂殖酵母和酵母/克鲁维酵母的谱系发生了重大变化。我们没有观察到呼吸代谢的特殊适应共同的所有致病物种。
Oxidative phosphorylation is central to the energy metabolism of the cell. Due to adaptation to different life-styles and environments, fungal species have shaped their respiratory pathways in the course of evolution. To identify the main mechanisms behind the evolution of respiratory pathways, we conducted a phylogenomics survey of oxidative phosphorylation components in the genomes of sixty fungal species. Besides clarifying orthology and paralogy relationships among respiratory proteins, our results reveal three parallel losses of the entire complex I, two of which are coupled to duplications in alternative dehydrogenases. Duplications in respiratory proteins have been common, affecting 76% of the protein families surveyed. We detect several instances of paralogs of genes coding for subunits of respiratory complexes that have been recruited to other multi-protein complexes inside and outside the mitochondrion, emphasizing the role of evolutionary tinkering. Processes of gene loss and gene duplication followed by functional divergence have been rampant in the evolution of fungal respiration. Overall, the core proteins of the respiratory pathways are conserved in most lineages, with major changes affecting the lineages of microsporidia, Schizosaccaromyces and Saccharomyces/Kluyveromyces due to adaptation to anaerobic life-styles. We did not observe specific adaptations of the respiratory metabolism common to all pathogenic species.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
12.3
作者：
Gabaldón T
通讯作者：
Gabaldón T
影响因子：
3.5
作者：
De Grassi, Anna;Lanave, Cecilia;Saccone, Cecilia
通讯作者：
Saccone, Cecilia
影响因子：
3
作者：
Lavin, Jose L.;Oguiza, Jose A.;Pisabarro, Antonio G.
通讯作者：
Pisabarro, Antonio G.
影响因子：
14.9
作者：
Christie, KR;Weng, S;Cherry, JM
通讯作者：
Cherry, JM