Development of a non-invasive method for skin cholesterol detection: pre-clinical assessment in atherosclerosis screening.
Development of a non-invasive method for skin cholesterol detection: pre-clinical assessment in atherosclerosis screening.
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DOI：
10.1186/s12938-021-00889-1
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发表时间：
2021-06-01
影响因子：
3.9
通讯作者：
Dong M
中科院分区：
文献类型：
--
作者：
Ni J;Hong H;Zhang Y;Tang S;Han Y;Fang Z;Zhang Y;Zhou N;Wang Q;Liu Y;Li Z;Wang Y;Dong M
建立一种高精度、非侵入性的心血管疾病评估方法是必不可少的。皮肤胆固醇是一种评估动脉粥样硬化风险的新标记物，可作为动脉粥样硬化风险早期评估的独立危险因素。提出了一种基于吸收光谱的非侵入性皮肤胆固醇检测方法。检测试剂专门与皮肤胆固醇结合，与指示剂反应生成有色产品，通过非侵入性技术检测有色产品的吸收光谱信息，即可获得皮肤胆固醇含量。用气相色谱检测从皮肤中提取的胆固醇，以验证非侵入性检测方法的准确性和可靠性。将342例受试者分为正常组(n = 115例)、疾病组(n = 110例)和危险组(n = 117例)。所有受试者都接受了非侵入性皮肤胆固醇测试。用受试者-操作特征(ROC)曲线分析测量值的诊断准确性。所提出的方法能够识别含有梯度浓度胆固醇的猪皮。无创检测法测定值与气相色谱测定值呈显著正相关(r = 0.9074，n = 73，p <； 0.001)。布兰德-阿尔特曼偏倚为−72.78 ± 20.03，95%的符合度范围为−112.05~−33.51，均在预先指定的临床无意义范围内。我们进一步评估了动脉粥样硬化患者和危险人群以及正常组的方法，患者和危险动脉粥样硬化组的皮肤胆固醇含量高于正常组(均P <； 0.001)。区分正常/疾病组的ROC曲线下面积为0.8642(95%可信区间0.8138~0.9146)，区分正常/危险组的ROC曲线下面积为0.8534(95%可信区间0.8034~0.9034)。该方法能够检测不同浓度的皮肤胆固醇。这种非侵入性皮肤胆固醇检测系统可能被用作动脉粥样硬化筛查的风险评估工具，特别是在大量人群中。网上版载有补充材料，可在10.1186/s12938-021-00889-1查阅。
Establishing a high-accuracy and non-invasive method is essential for evaluating cardiovascular disease. Skin cholesterol is a novel marker for assessing the risk of atherosclerosis and can be used as an independent risk factor of early assessment of atherosclerotic risk. We propose a non-invasive skin cholesterol detection method based on absorption spectroscopy. Detection reagents specifically bind to skin cholesterol and react with indicator to produce colored products, the skin cholesterol content can be obtained through absorption spectrum information on colored products detected by non-invasive technology. Gas chromatography is used to measure cholesterol extracted from the skin to verify the accuracy and reliability of the non-invasive test method. A total of 342 subjects were divided into normal group (n = 115), disease group (n = 110) and risk group (n = 117). All subjects underwent non-invasive skin cholesterol test. The diagnostic accuracy of the measured value was analyzed by receiver-operating characteristic (ROC) curve. The proposed method is able to identify porcine skin containing gradient concentration of cholesterol. The values measured by non-invasive detection method were significantly correlated with gas chromatography measured results (r = 0.9074, n = 73, p < 0.001). Bland–Altman bias was − 72.78 ± 20.03 with 95% limits of agreement − 112.05 to − 33.51, falling within the prespecified clinically non-significant range. We further evaluated the method of patients with atherosclerosis and risk population as well as normal group, patients and risk atherosclerosis group exhibited higher skin cholesterol content than normal group (all P < 0.001). The area under the ROC curve for distinguishing Normal/Disease group was 0.8642 (95% confidence interval, 0.8138 to 0.9146), meanwhile, the area under the ROC curve for distinguishing Normal/Risk group was 0.8534 (95% confidence interval, 0.8034 to 0.9034). The method demonstrated its capability of detecting different concentration of skin cholesterol. This non-invasive skin cholesterol detection system may potentially be used as a risk assessment tool for atherosclerosis screening, especially for a large population. The online version contains supplementary material available at 10.1186/s12938-021-00889-1.
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影响因子：
2.8
作者：
Stein, James H.;Tzou, Wendy S.;Davidson, Michael H.
通讯作者：
Davidson, Michael H.
影响因子：
2.7
作者：
Logan, Ian T.;Logan, Richard A.
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影响因子：
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作者：
Luister, Alexandra;Schoett, Hans Frieder;Weingaertner, Oliver
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影响因子：
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作者：
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DOI：
10.1364/josaa.377121
发表时间：
2020-03-01
影响因子：
1.9
作者：
Guan, Anna;Richardson, Steven;Hinckley, Steven
通讯作者：
Hinckley, Steven