Fungal extracts detected in eosinophilic chronic rhinosinusitis induced cytokines from the nasal polyp cells.
Fungal extracts detected in eosinophilic chronic rhinosinusitis induced cytokines from the nasal polyp cells.
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DOI：
10.1002/lary.24655
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发表时间：
2014-09
期刊：
影响因子：
2.6
通讯作者：
Ikeda, Katsuhisa
中科院分区：
文献类型：
--
作者：
Hirotsu, Mikio;Shiozawa, Akihito;Ono, Noritsugu;Miwa, Masato;Kikuchi, Ken;Ikeda, Katsuhisa
真菌在慢性鼻窦炎（CRS）中的作用仍有争议。本研究旨在检测和鉴定嗜酸性和非嗜酸性CRS鼻息肉组织中的真菌种类，并确定真菌抗原在细胞因子产生中的作用。前瞻性研究。从CRS患者中收集了35例鼻息肉标本，并使用培养、组织学和聚合酶链反应分析进行真菌检查。应用多重免疫分析法测定了真菌提取物刺激分散的鼻息肉细胞（DNPCs）分泌14种细胞因子。在鼻息肉匀浆培养物中没有微生物生长（包括真菌）。此外，所有鼻息肉的Grocott甲胺银染色均未显示真菌体。35例鼻息肉标本中有16例真菌DNA扩增。蝶窦粘膜均未检出真菌DNA。真菌DNA阳性的鼻息肉组织中嗜酸性粒细胞的数量明显高于真菌DNA阴性的鼻息肉组织（P <0.01）。真菌提取物增强了DNPC分泌的嗜酸性粒细胞相关细胞因子如白细胞介素（IL）-5、IL-13、IL-17 A和RANTES（受活化正常T细胞表达和分泌的调节）以及促炎细胞因子如IL-6、IL-8、肿瘤坏死因子-α和粒细胞-巨噬细胞集落刺激因子。本研究提供了直接的证据支持，真菌元素修改嗜酸性CRS鼻息肉的炎症反应。证据等级：NA
The role of fungi in chronic rhinosinusitis (CRS) is still controversial. The present study was conducted to detect and identify fungal species from the nasal polyp tissues of eosinophilic and noneosinophilic CRS, and to determine the role of fungal antigens in cytokine production. Prospective study. Thirty-five specimens of nasal polyps were collected from patients with CRS and examined for fungus using culture, histology, and polymerase chain reaction analysis. The secretion of 14 cytokines stimulated by fungal extracts using dispersed nasal polyp cells (DNPCs) was determined by multiplex immunoassay. There was no microbiological growth (including fungus) in the cultures of homogenized nasal polyps. Furthermore, Grocott methanamine silver staining for all nasal polyps showed no fungal bodies. Sixteen of 35 samples of the nasal polyps showed amplification of fungal DNA. In none of the mucosa of the sphenoid sinus was fungal DNA detected. The number of eosinophils in the nasal polyps in which fungal DNA was detected was significantly higher than in the nasal polyps in which fungal DNA was not detected (P < .01). The extract of fungus enhanced the secretion of eosinophil-associated cytokines such as interleukine (IL)-5, IL-13, IL-17A, and RANTES (regulated on activation normal T-cell expressed and secreted), and proinflammatory cytokines such as IL-6, IL-8, tumor necrosis factor-α, and granulocyte-macrophage colony-stimulating factor from DNPCs. The present study offers direct evidence supporting that fungal elements modify the inflammatory response in the nasal polyps of eosinophilic CRS. Level of Evidence: NA
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影响因子：
3.4
作者：
Healy, David Y.;Leid, Jeff G.;Hunsaker, Darrell H.
通讯作者：
Hunsaker, Darrell H.
影响因子：
12.4
作者：
Mansson, A.;Bogefors, J.;Cardell, L. O.
通讯作者：
Cardell, L. O.
影响因子：
2.6
作者：
Murr, Andrew H.;Goldberg, Andrew N.;Vesper, Stephen
通讯作者：
Vesper, Stephen
影响因子：
7.2
作者：
Fokkens, Wytske J.;Lund, Valerie J.;Zwetsloot, Casper P.
通讯作者：
Zwetsloot, Casper P.
影响因子：
9.4
作者：
Kurtzman, CP;Robnett, CJ
通讯作者：
Robnett, CJ