Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds.
Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds.
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DOI：
10.1117/1.3130345
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发表时间：
2009-05
影响因子：
3.5
通讯作者：
Wang RK
中科院分区：
文献类型：
--
作者：
Jia Y;Bagnaninchi PO;Yang Y;Haj AE;Hinds MT;Kirkpatrick SJ;Wang RK
在 3 维细胞培养环境中建立灌注速率和流体剪切应力之间的关系是组织工程师面临的一项持续且具有挑战性的任务。在这项研究中，我们探索多普勒光学相干断层扫描（DOCT）作为一种潜在的成像工具，用于原位监测多孔壳聚糖支架内的局部流体流动剖面。根据测量的流体流动剖面，可以评估流体剪切应力。我们检查了低孔隙率和高孔隙率壳聚糖支架内的局部流体流动和剪切应力，这些支架受到 0.5 ml·min-1 的恒定输入流速的影响。 DOCT 结果表明，微孔中的流体流动和剪切应力行为强烈依赖于支架内微孔的互连性、孔隙率和孔的尺寸。对于所检查的低孔隙率和高孔隙率壳聚糖支架，测得的局部流体流动和剪切应力因微孔而异，平均剪切应力分别为0.49±0.3 dyn·cm-2和0.38±0.2 dyn·cm-2。此外，我们还表明，通过结合从 DOCT 获得的 3 维结构和流动图像的分析，可以量化支架的孔隙率和互连性。
Establishing a relationship between perfusion rate and fluid shear stress in a 3-dimensional cell culture environment is an ongoing and challenging task faced by tissue engineers. In this study, we explore Doppler optical coherence tomography (DOCT) as a potential imaging tool for in situ monitoring of local fluid flow profiles inside porous chitosan scaffolds. From the measured fluid flow profiles, the fluid shear stresses are evaluated. We examine the localized fluid flow and shear stress within low and high porosity chitosan scaffolds, which are subjected to a constant input flow rate of 0.5 ml·min-1. The DOCT results show that the behaviour of the fluid flow and shear stress in micropores is strongly dependent on the micropore interconnectivity, porosity, and size of pores within the scaffold. For low porosity and high porosity chitosan scaffolds examined, the measured local fluid flow and shear stress varied from micropore to micropore with a mean shear stress of 0.49±0.3 dyn·cm-2 and 0.38±0.2 dyn·cm-2, respectively. In addition, we show that the scaffold’s porosity and interconnectivity can be quantified by combining analyses of the 3-dimensional structural and flow images obtained from DOCT.
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影响因子：
2.4
作者：
Donahue, TLH;Haut, TR;Jacobs, CR
通讯作者：
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影响因子：
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