An automated microfluidic multiplexer for fast delivery of C. elegans populations from multiwells.
An automated microfluidic multiplexer for fast delivery of C. elegans populations from multiwells.
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DOI：
10.1371/journal.pone.0074480
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ben-Yakar A
中科院分区：
文献类型：
--
作者：
Ghorashian N;Gökçe SK;Guo SX;Everett WN;Ben-Yakar A
自动化生物分选平台，包括最近开发的微流体装置，可以实现并加速小动物模型的高通量和/或高分辨率生物分析。然而，将不同的生物种群运送到这些系统中耗时，这是执行大规模筛选的主要瓶颈。目前的人口输送策略依赖于传统井板通过定期暴露在空气中的油管进行吸出，这导致了一些缺点：1)气泡引入样品，干扰下游系统的分析，2)由于增加气泡清洗步骤而耗费大量时间，3)需要复杂的机械系统来操纵井板位置。为了解决这些问题，我们开发了一种多井格式的微流控平台，可以通过多路阀控制从芯片上的井中输送多种不同的动物种群。这种人口输送芯片可以作为一个相对简单的设置的一部分自动运行，不需要任何典型的板处理系统的主要机械运动部件来处理给定的井。我们演示了将16种不同的秀丽隐杆线虫种群自动连续输送到单个出口，而不会向样品引入任何气泡，造成交叉污染或损害动物。该设备在4.7秒内将90%以上的人口预加载到给定的井中；比目前使用的交付方式快一个数量级。这个平台可以潜在地处理其他类似大小的模式生物，如斑马鱼和果蝇幼虫或细胞微菌落。该设备的架构和微通道尺寸允许简单的扩展，以处理更多的人口。
Automated biosorter platforms, including recently developed microfluidic devices, enable and accelerate high-throughput and/or high-resolution bioassays on small animal models. However, time-consuming delivery of different organism populations to these systems introduces a major bottleneck to executing large-scale screens. Current population delivery strategies rely on suction from conventional well plates through tubing periodically exposed to air, leading to certain disadvantages: 1) bubble introduction to the sample, interfering with analysis in the downstream system, 2) substantial time drain from added bubble-cleaning steps, and 3) the need for complex mechanical systems to manipulate well plate position. To address these concerns, we developed a multiwell-format microfluidic platform that can deliver multiple distinct animal populations from on-chip wells using multiplexed valve control. This Population Delivery Chip could operate autonomously as part of a relatively simple setup that did not require any of the major mechanical moving parts typical of plate-handling systems to address a given well. We demonstrated automatic serial delivery of 16 distinct C. elegans worm populations to a single outlet without introducing any bubbles to the samples, causing cross-contamination, or damaging the animals. The device achieved delivery of more than 90% of the population preloaded into a given well in 4.7 seconds; an order of magnitude faster than delivery modalities in current use. This platform could potentially handle other similarly sized model organisms, such as zebrafish and drosophila larvae or cellular micro-colonies. The device’s architecture and microchannel dimensions allow simple expansion for processing larger numbers of populations.
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DOI：
10.1073/pnas.79.21.6603
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
JOHNSON, TE;WOOD, WB
通讯作者：
WOOD, WB
影响因子：
6.1
作者：
Hulme, S. Elizabeth;Shevkoplyas, Sergey S.;Whitesides, George M.
通讯作者：
Whitesides, George M.
影响因子：
6.1
作者：
Chung K;Zhan M;Srinivasan J;Sternberg PW;Gong E;Schroeder FC;Lu H
通讯作者：
Lu H
影响因子：
7.4
作者：
Singhal, Anupam;Haynes, Charles A.;Hansen, Carl L.
通讯作者：
Hansen, Carl L.
影响因子：
6.1
作者：
Lockery SR;Hulme SE;Roberts WM;Robinson KJ;Laromaine A;Lindsay TH;Whitesides GM;Weeks JC
通讯作者：
Weeks JC