Enhanced and Stem-Cell-Compatible Effects of Nature-Inspired Antimicrobial Nanotopography and Antimicrobial Peptides to Combat Implant-Associated Infection.
Enhanced and Stem-Cell-Compatible Effects of Nature-Inspired Antimicrobial Nanotopography and Antimicrobial Peptides to Combat Implant-Associated Infection.
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自然启发的抗菌纳米形貌和抗菌肽对抗植入物相关感染的增强和干细胞相容性作用。
DOI：
10.1021/acsanm.2c04913
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发表时间：
2023-02-24
影响因子：
5.9
通讯作者：
Su, Bo
中科院分区：
文献类型：
--
作者：
Ishak, Mohd Irill;Eales, Marcus;Damiati, Laila;Liu, Xiayi;Jenkins, Joshua;Dalby, Matthew J.;Nobbs, Angela H.;Ryadnov, Maxim G.;Su, Bo
受自然启发的抗菌表面和抗菌肽（AMPs）已经成为对抗植入物相关感染的有希望的策略。本研究通过物理吸附将一种仿生抗菌肽功能化到纳米尖（NS）表面，目的是使其逐渐释放到局部环境中，从而增强对细菌生长的抑制作用。与纳米形貌相比，在对照平面上吸附的肽表现出不同的释放动力学，但两种表面都表现出优异的抗菌性能。微摩尔多肽功能化抑制了大肠杆菌在平面上的生长，抑制了金黄色葡萄球菌在NS表面的生长，抑制了表皮葡萄球菌在NS表面的生长。基于这些数据，我们提出了一种增强的抗菌机制，即AMPs可以使细菌细胞膜更容易受到纳米尖刺的影响，并且纳米尖刺引起的膜变形可以增加AMPs插入膜的表面积。综合起来，这些作用增强了杀菌活性。由于功能化纳米结构与干细胞具有高度的生物相容性，因此它们有望成为下一代抗菌植入物表面的候选材料。
Nature-inspired antimicrobial surfaces and antimicrobial peptides (AMPs) have emerged as promising strategies to combat implant-associated infections. In this study, a bioinspired antimicrobial peptide was functionalized onto a nanospike (NS) surface by physical adsorption with the aim that its gradual release into the local environment would enhance inhibition of bacterial growth. Peptide adsorbed on a control flat surface exhibited different release kinetics compared to the nanotopography, but both surfaces showed excellent antibacterial properties. Functionalization with peptide at micromolar concentrations inhibited Escherichia coli growth on the flat surface, Staphylococcus aureus growth on the NS surface, and Staphylococcus epidermidis growth on both the flat and NS surfaces. Based on these data, we propose an enhanced antibacterial mechanism whereby AMPs can render bacterial cell membranes more susceptible to nanospikes, and the membrane deformation induced by nanospikes can increase the surface area for AMPs membrane insertion. Combined, these effects enhance bactericidal activity. Since functionalized nanostructures are highly biocompatible with stem cells, they make promising candidates for next generation antibacterial implant surfaces.
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影响因子：
4.6
作者：
Diu T;Faruqui N;Sjöström T;Lamarre B;Jenkinson HF;Su B;Ryadnov MG
通讯作者：
Ryadnov MG
影响因子：
10.8
作者：
Bright, Richard;Hayles, Andrew;Vasilev, Krasimir
通讯作者：
Vasilev, Krasimir
影响因子：
16.6
作者：
Jenkins, J.;Mantell, J.;Su, B.
通讯作者：
Su, B.
影响因子：
9.9
作者：
Elbourne, Aaron;Crawford, Russell J.;Ivanova, Elena P.
通讯作者：
Ivanova, Elena P.
影响因子：
9.9
作者：
Ishak, Mohd I.;Dobryden, Illia;Su, Bo
通讯作者：
Su, Bo