Sli15 associates with the ipl1 protein kinase to promote proper chromosome segregation in Saccharomyces cerevisiae.
Sli15 associates with the ipl1 protein kinase to promote proper chromosome segregation in Saccharomyces cerevisiae.
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DOI：
10.1083/jcb.145.7.1381
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发表时间：
1999-06-28
期刊：
影响因子：
--
通讯作者：
Chan CS
中科院分区：
文献类型：
--
作者：
Kim JH;Kang JS;Chan CS
保守的Ipl1蛋白激酶对于酿酒酵母的染色体分离和细胞活力至关重要。它的人类同源物与多种癌症的肿瘤发生有关。我们在这里表明，姐妹染色单体彼此分离并没有正确地分离到ipl1-2细胞的相反两极。染色体分离失败通常与纺锤体极相关的Nuf2-GFP蛋白的异常分布有关，这表明在ipl1突变细胞中，潜在的纺锤体极缺陷与染色体错误分离之间存在联系。一小部分ipl1-2细胞在核迁移或双极纺锤体形成方面也存在缺陷。Ipl1可能直接与体内新的必需的Sli15蛋白结合，这两种蛋白都定位于有丝分裂纺锤体。条件sli15突变细胞的细胞学表型与ipl1细胞非常相似，ipl1-2突变与sli15突变表现出合成致死性遗传相互作用。Sli15突变型表型，像ipl1突变型表型一样，被减少蛋白磷酸酶1功能的扰动部分抑制。这些遗传和生化研究表明，Sli15与Ipl1结合，促进其在染色体分离中的功能。
The conserved Ipl1 protein kinase is essential for proper chromosome segregation and thus cell viability in the budding yeast Saccharomyces cerevisiae. Its human homologue has been implicated in the tumorigenesis of diverse forms of cancer. We show here that sister chromatids that have separated from each other are not properly segregated to opposite poles of ipl1-2 cells. Failures in chromosome segregation are often associated with abnormal distribution of the spindle pole–associated Nuf2-GFP protein, thus suggesting a link between potential spindle pole defects and chromosome missegregation in ipl1 mutant cells. A small fraction of ipl1-2 cells also appears to be defective in nuclear migration or bipolar spindle formation. Ipl1 associates, probably directly, with the novel and essential Sli15 protein in vivo, and both proteins are localized to the mitotic spindle. Conditional sli15 mutant cells have cytological phenotypes very similar to those of ipl1 cells, and the ipl1-2 mutation exhibits synthetic lethal genetic interaction with sli15 mutations. sli15 mutant phenotype, like ipl1 mutant phenotype, is partially suppressed by perturbations that reduce protein phosphatase 1 function. These genetic and biochemical studies indicate that Sli15 associates with Ipl1 to promote its function in chromosome segregation.
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影响因子：
7.8
作者：
GOH, PY;KILMARTIN, JV
通讯作者：
KILMARTIN, JV
DOI：
10.1083/jcb.118.1.109
发表时间：
1992-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hoyt MA;He L;Loo KK;Saunders WS
通讯作者：
Saunders WS
影响因子：
16
作者：
Cho, RJ;Campbell, MJ;Davis, RW
通讯作者：
Davis, RW
影响因子：
11.4
作者：
Andrésson, T;Ruderman, JV
通讯作者：
Ruderman, JV
影响因子：
64.5
作者：
Guacci, V;Koshland, D;Strunnikov, A
通讯作者：
Strunnikov, A