Avian Primordial Germ Cells Are Bipotent for Male or Female Gametogenesis.
Avian Primordial Germ Cells Are Bipotent for Male or Female Gametogenesis.
复制标题
DOI：
10.3389/fcell.2021.726827
复制
发表时间：
2021
影响因子：
5.5
通讯作者：
McGrew MJ
中科院分区：
文献类型：
--
作者：
Ballantyne M;Taylor L;Hu T;Meunier D;Nandi S;Sherman A;Flack B;Henshall JM;Hawken RJ;McGrew MJ
In birds, males are the homogametic sex (ZZ) and females are the heterogametic sex (ZW). Here, we investigate the role of chromosomal sex and germ cell competition on avian germ cell differentiation. We recently developed genetically sterile layer cockerels and hens for use as surrogate hosts for primordial germ cell (PGC) transplantation. Using in vitro propagated and cryopreserved PGCs from a pedigree Silkie broiler breed, we now demonstrate that sterile surrogate layer hosts injected with same sex PGCs have normal fertility and produced pure breed Silkie broiler offspring when directly mated to each other in Sire Dam Surrogate mating. We found that female sterile hosts carrying chromosomally male (ZZ) PGCs formed functional oocytes and eggs, which gave rise to 100% male offspring after fertilization. Unexpectedly, we also observed that chromosomally female (ZW) PGCs carried by male sterile hosts formed functional spermatozoa and produced viable offspring. These findings demonstrate that avian PGCs are not sexually restricted for functional gamete formation and provide new insights for the cryopreservation of poultry and other bird species using diploid stage germ cells.
登录
查看更多内容
影响因子：
3.7
作者：
Macdonald J;Glover JD;Taylor L;Sang HM;McGrew MJ
通讯作者：
McGrew MJ
影响因子：
2.5
作者：
Tagami, Takahiro;Kagami, Hiroshi;Nirasawa, Keijiro
通讯作者：
Nirasawa, Keijiro
影响因子：
2.4
作者：
Panda SK;McGrew MJ
通讯作者：
McGrew MJ
影响因子：
2.7
作者：
Dranow, Daniel B.;Tucker, Richard P.;Draper, Bruce W.
通讯作者：
Draper, Bruce W.
影响因子：
16.6
作者：
Ballantyne M;Woodcock M;Doddamani D;Hu T;Taylor L;Hawken RJ;McGrew MJ
通讯作者：
McGrew MJ