Diverse osteoclastogenesis of bone marrow from mandible versus long bone.
Diverse osteoclastogenesis of bone marrow from mandible versus long bone.
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DOI：
10.1902/jop.2013.130376
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发表时间：
2014-06
影响因子：
4.3
通讯作者：
Tetradis S
中科院分区：
文献类型：
--
作者：
Chaichanasakul T;Kang B;Bezouglaia O;Aghaloo TL;Tetradis S
下颌骨（MB）和上颌骨具有独特的代谢和功能特性，并表现出对稳态，机械，激素和发育刺激的离散反应。骨髓基质细胞（BMSC）的成骨潜能在MB与长骨（LB）之间存在差异。此外，MB与LB衍生的破骨细胞（OC）具有不同的功能特性。在这里，我们探讨了破骨细胞的潜力，大鼠MB与LB骨髓在体外和体内的基础和刺激条件下。将大鼠MB和LB的骨髓在成骨细胞或成骨细胞分化培养基中培养。进行抗酒石酸酸性磷酸酶（TRAP）染色、再吸收陷窝测定和实时PCR。此外，在动物体内植入渗透微型泵，分离下颌骨和胫骨，并测量多核细胞（MNCs）。与用RANKL和M-CSF分化的LB骨髓培养物相比，MB产生更多的TRAP+多核细胞（MNC）和更大的吸收面积。为了探索MB与LB BMSC支持破骨细胞生成，将融合的BMSC与甲状旁腺激素（PTH）、1α，25-二羟基维生素D3（1，25 D3）或PTH+1，25 D3培养。1，25 D3或PTH+1，25 D3处理的LB BMSCs表达RANKL和OPG mRNA，RANKL/OPG比值增加。当用PTH+1，25 D3培养全骨髓时，在LB培养物中比在MB培养物中观察到更多的TRAP+ MNC。最后，大鼠输注PTH+1，25 D3和MB与胫骨MNCs进行测量。激素刺激增加MB和胫骨中的破骨细胞生成。然而，在基础和激素刺激下，胫骨中观察到的TRAP+ MNC数量高于MB。总的来说，我们的数据说明了MB与LB骨髓的破骨细胞生成潜力和OC数量的差异。
Mandible (MB) and maxilla possess unique metabolic and functional properties and demonstrate discrete responses to homeostatic, mechanical, hormonal and developmental stimuli. Osteogenic potential of bone marrow stromal cells (BMSCs) differs between MB versus long bones (LB). Furthermore, MB versus LB derived osteoclasts (OCs) have disparate functional properties. Here, we explored the osteoclastogenic potential of rat MB versus LB marrow in vitro and in vivo under basal and stimulated conditions. Bone marrow from rat MB and LB was cultured in osteoblastic or osteoclastic differentiation media. Tartrate resistant acid phosphatase (TRAP) staining, resorption pit assays, and real-time PCR were performed. Additionally, osmotic mini-pumps were implanted in animals, mandibles and tibiae were isolated and multinucleated cells (MNCs) were measured. MB versus LB marrow cultures differentiated with RANKL and M-CSF produced more TRAP+ multinucleated cells (MNCs) and greater resorptive area. To explore MB versus LB BMSC supported osteoclastogenesis, confluent BMSCs were cultured with parathyroid hormone (PTH), 1α,25-dihydroxyvitaminD3 (1,25D3), or PTH+1,25D3. 1,25D3 or PTH+1,25D3 treated LB BMSCs expressed significantly higher RANKL and lower OPG mRNA and increased RANKL:OPG ratio. When whole marrow was cultured with PTH+1,25D3, more TRAP+ MNCs were seen in LB versus MB cultures. Ultimately, rats were infused with PTH+1,25D3 and MB versus tibia MNCs were measured. Hormonal stimulation increased osteoclastogenesis in both MB and tibia. However, higher TRAP+ MNC numbers were observed in tibia versus MB under basal and hormonal stimulation. Collectively, our data illustrate differences both on osteoclastogenic potential and OC numbers of MB versus LB marrow.
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影响因子：
6.2
作者：
Mavropoulos, Anestis;Rizzoli, Rene;Ammann, Patrick
通讯作者：
Ammann, Patrick
影响因子：
4
作者：
MARTIN, TJ;NG, KW
通讯作者：
NG, KW
影响因子：
4.1
作者：
Akintoye, Sunday O.;Lam, Thanh;Robey, Pamela G.
通讯作者：
Robey, Pamela G.
影响因子：
2.3
作者：
de Smit, M. J.;Brouwer, E.;van Winkelhoff, A. J.
通讯作者：
van Winkelhoff, A. J.
DOI：
10.1006/bbrc.1999.0524
发表时间：
1999-04-21
影响因子：
3.1
作者：
Nagai, M;Sato, N
通讯作者：
Sato, N