Natural variation of physiological traits, molecular markers, and chlorophyll catabolic genes associated with heat tolerance in perennial ryegrass accessions.
Natural variation of physiological traits, molecular markers, and chlorophyll catabolic genes associated with heat tolerance in perennial ryegrass accessions.
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多年生黑麦草种质中与耐热性相关的生理性状、分子标记和叶绿素分解代谢基因的自然变异
DOI：
10.1186/s12870-020-02695-8
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发表时间：
2020-11-16
影响因子：
5.3
通讯作者：
Huang B
中科院分区：
文献类型：
--
作者：
Zhang J;Li H;Jiang Y;Li H;Zhang Z;Xu Z;Xu B;Huang B
寒季禾本科植物耐热性遗传多样性及其相关性状的鉴定对提高其耐热性具有重要意义。本研究的目的是确定多年生黑麦草（Lolium perenneL）耐热性与表型和生理性状相关的遗传变异，并鉴定与耐热性相关的分子标记。结果98份材料分别在35/30°C昼/夜高温胁迫和25/20°C最佳生长温度条件下培养24 d。采用主成分分析（PCA）对8个表型和生理性状进行了综合耐热性排序。其中，电解质泄漏量（EL）、叶绿素含量（Chl）、相对含水量（RWC）与耐热性PCA排序具有较高的相关系数（分别为- 0.858、0.769和0.764）。热胁迫下，4个Chl分解代谢基因（CCGs） lpnyc1、LpNOL、LpSGR和lppph在热敏黑麦草中的表达量显著高于耐热黑麦草。此外，利用66对SSR标记对主成分分析结果进行关联分析。黑麦草群体结构可分为3个聚类，聚类C比聚类A和聚类b耐热性较好，与上述性状显著相关的SSR标记显著（R2> 0.05,p< 0.01）。，包括位于第4染色体上与Chl含量相关的2对标记和与EL含量相关的4对标记。结论该结果不仅鉴定出了多年生黑麦草耐热育种有用的EL、Chl含量、RWC等生理参数及其相关SSR标记，还揭示了Chl分解代谢在黑麦草耐热育种中的作用。这对多年生黑麦草和其他寒季禾本科植物的耐热育种和耐热机理研究具有重要意义。
BackgroundIdentification of genetic diversity in heat tolerance and associated traits is of great importance for improving heat tolerance in cool-season grass species. The objectives of this study were to determine genetic variations in heat tolerance associated with phenotypic and physiological traits and to identify molecular markers associated with heat tolerance in a diverse collection of perennial ryegrass (Lolium perenneL.).ResultsPlants of 98 accessions were subjected to heat stress (35/30 °C, day/night) or optimal growth temperature (25/20 °C) for 24 d in growth chambers. Overall heat tolerance of those accessions was ranked by principal component analysis (PCA) based on eight phenotypic and physiological traits. Among these traits, electrolyte leakage (EL), chlorophyll content (Chl), relative water content (RWC) had high correlation coefficients (− 0.858, 0.769, and 0.764, respectively) with the PCA ranking of heat tolerance. We also found expression levels of four Chl catabolic genes (CCGs), includingLpNYC1,LpNOL,LpSGR, andLpPPH, were significant higher in heat sensitive ryegrass accessions then heat tolerant ones under heat stress. Furthermore, 66 pairs of simple sequence repeat (SSR) markers were used to perform association analysis based on the PCA result. The population structure of ryegrass can be grouped into three clusters, and accessions in cluster C were relatively more heat tolerant than those in cluster A and B. SSR markers significantly associated with above-mentioned traits were identified (R2> 0.05,p< 0.01)., including two pairs of markers located on chromosome 4 in association with Chl content and another four pairs of markers in association with EL.ConclusionThe result not only identified useful physiological parameters, including EL, Chl content, and RWC, and their associated SSR markers for heat-tolerance breeding of perennial ryegrass, but also highlighted the involvement of Chl catabolism in ryegrass heat tolerance. Such knowledge is of significance for heat-tolerance breeding and heat tolerance mechanisms in perennial ryegrass as well as in other cool-season grass species.
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影响因子：
2.3
作者：
Jespersen, David;Merewitz, Emily;Huang, Bingru
通讯作者：
Huang, Bingru
影响因子：
4.9
作者：
Munns, R;Husain, S;Hare, RA
通讯作者：
Hare, RA
影响因子：
2.7
作者：
Dumsday, JL;Smith, KF;Jones, ES
通讯作者：
Jones, ES
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
4.2
作者：
King, J.;Thorogood, D.;King, I. P.
通讯作者：
King, I. P.