Gut and lung microbiome profiles in pregnant mice.
Gut and lung microbiome profiles in pregnant mice.
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怀孕小鼠的肠道和肺微生物组。
DOI：
10.3389/fmicb.2022.946779
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发表时间：
2022
影响因子：
5.2
通讯作者：
Gonzalez-Juarbe, Norberto
中科院分区：
文献类型：
--
作者：
Wiscovitch-Russo, Rosana;Taal, Aji Mary;Kuelbs, Claire;Oldfield, Lauren M.;Ramar, MohanKumar;Singh, Harinder;Fedulov, Alexey V.;Gonzalez-Juarbe, Norberto
近年来，微生物组研究已经从胃肠道扩展到以前被认为是非细菌的其他宿主部位，如肺部。然而，怀孕对肺部和肠道微生物群的影响尚不清楚。在这里，我们检查了妊娠14天小鼠肠道和肺部微生物组的变化。采集妊娠和非妊娠雌性BALB/c小鼠肺组织和粪便样本，分离、扩增DNA，测序细菌特异性V4 16S rRNA基因。使用内部生物信息学管道，我们使用粪便和肺组织样本评估每个器官的微生物组成。结果表明，妊娠小鼠粪便中毛缕菌科和乳酸杆菌科含量较高。同样，乳酸杆菌科在怀孕小鼠的肺中占主导地位。然而，链球菌科在未怀孕小鼠的肺部占主导地位，而怀孕小鼠的微生物丰度较低。一项排列测试表明，怀孕对肺部和粪便微生物组的变异有显著影响。同时，我们估计在粪便和肺部数据之间共有49%的检测到的操作分类单位。在从肺部数据集中去除常见的粪便相关细菌后，未检测到孕妇和非孕妇肺部微生物群落之间的微生物丰度差异。因此，怀孕会导致肺部和粪便微生物群的变化，但不会影响肺部特有的微生物群。
In recent years, microbiome research has expanded from the gastrointestinal tract to other host sites previously thought to be abacterial such as the lungs. Yet, the effects of pregnancy in the lung and gut microbiome remains unclear. Here we examined the changes in the gut and lung microbiome in mice at 14 days of gestation. Lung tissue and stool samples were collected from pregnant and non-pregnant female BALB/c mice, DNA was isolated, amplified, and bacterial specific V4 16S rRNA gene was sequenced. Using an in-house bioinformatic pipeline we assessed the microbial composition of each organ using stool and lung tissue samples. The stool data showed that Lachnospiraceae and Lactobacillaceae were more abundant in the pregnant mice. Likewise, Lactobacillaceae were dominant in the lungs of pregnant mice. However, Streptococcaceae were dominant in the lungs of non-pregnant mice with a low microbial abundance in the pregnant mice. A permutation test showed that pregnancy significantly contributes to the variance in both the lung and stool microbiome. At the same time, we estimate that 49% of the total detected operational taxonomic units were shared between the stool and lung data. After removing common stool-associated bacteria from the lung dataset, no microbial differential abundance was detected between the pregnant and non-pregnant lung microbial community. Thus, pregnancy contributes to variance to the lung and stool microbiome but not in the unique lung microbiota.
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影响因子：
8.3
作者：
Dickson, Robert P.;Erb-Downward, John R.;Curtis, Jeffrey L.
通讯作者：
Curtis, Jeffrey L.
影响因子：
3.7
作者：
Bogaert D;Keijser B;Huse S;Rossen J;Veenhoven R;van Gils E;Bruin J;Montijn R;Bonten M;Sanders E
通讯作者：
Sanders E
影响因子：
6.4
作者：
Cardona C;Lax S;Larsen P;Stephens B;Hampton-Marcell J;Edwardson CF;Henry C;Van Bonn B;Gilbert JA
通讯作者：
Gilbert JA
影响因子：
5.8
作者：
Edgar, Robert C.
通讯作者：
Edgar, Robert C.
影响因子：
4.6
作者：
Freire, Marcelo;Moustafa, Ahmed;Nelson, Karen E.
通讯作者：
Nelson, Karen E.