Conducting Polymer Scaffolds Based on Poly(3,4-ethylenedioxythiophene) and Xanthan Gum for Live-Cell Monitoring.
Conducting Polymer Scaffolds Based on Poly(3,4-ethylenedioxythiophene) and Xanthan Gum for Live-Cell Monitoring.
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基于聚合物（3,4-乙二甲噻吩）和黄原胶进行聚合物支架进行活细胞监测。
DOI：
10.1021/acsomega.8b00458
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发表时间：
2018-07-31
期刊：
影响因子：
4.1
通讯作者：
Mecerreyes D
中科院分区：
文献类型：
--
作者：
Del Agua I;Marina S;Pitsalidis C;Mantione D;Ferro M;Iandolo D;Sanchez-Sanchez A;Malliaras GG;Owens RM;Mecerreyes D
导电聚合物支架可以通过电刺激促进细胞生长，这对某些特定类型的细胞如神经元、肌肉细胞或心脏细胞是有利的。作为一个附加特性，它们的阻抗测量已被证明是监测支架内细胞生长的工具。在这项工作中，我们提出了基于聚（3,4-乙烯二氧噻吩）（PEDOT）：黄原胶的新型导电聚合物多孔支架，取代了众所周知的PEDOT：聚苯乙烯磺酸盐支架。这些新型支架结合了PEDOT的导电性和多糖黄原胶提供的机械支持和生物相容性。为此，首先，在多糖存在下对3,4-乙烯二氧噻吩进行氧化化学聚合，得到稳定的PEDOT：黄原胶水分散体。然后，通过一个简单的冷冻干燥过程，用这些分散体制备多孔支架。我们的研究结果表明，支架的孔隙率和力学性能是由初始PEDOT：多糖分散体的固体含量和配方调节的。支架的孔结构相互连接，孔径大小在10 ~ 150 μm之间，杨氏模量在10 ~ 45 kPa之间。这些支架成功地支持MDCK II eGFP和MDCK II LifeAct上皮细胞的三维细胞培养，实现了良好的细胞附着和非常高的孔覆盖率。有趣的是，通过测量合成的PEDOT支架的阻抗，可以监测细胞的生长情况。
Conducting polymer scaffolds can promote cell growth by electrical stimulation, which is advantageous for some specific type of cells such as neurons, muscle, or cardiac cells. As an additional feature, the measure of their impedance has been demonstrated as a tool to monitor cell growth within the scaffold. In this work, we present innovative conducting polymer porous scaffolds based on poly(3,4-ethylenedioxythiophene) (PEDOT):xanthan gum instead of the well-known PEDOT:polystyrene sulfonate scaffolds. These novel scaffolds combine the conductivity of PEDOT and the mechanical support and biocompatibility provided by a polysaccharide, xanthan gum. For this purpose, first, the oxidative chemical polymerization of 3,4-ethylenedioxythiophene was carried out in the presence of polysaccharides leading to stable PEDOT:xanthan gum aqueous dispersions. Then, by a simple freeze-drying process, porous scaffolds were prepared from these dispersions. Our results indicated that the porosity of the scaffolds and mechanical properties are tuned by the solid content and formulation of the initial PEDOT:polysaccharide dispersion. Scaffolds showed interconnected pore structure with tunable sizes ranging between 10 and 150 μm and Young’s moduli between 10 and 45 kPa. These scaffolds successfully support three-dimensional cell cultures of MDCK II eGFP and MDCK II LifeAct epithelial cells, achieving good cell attachment with very high degree of pore coverage. Interestingly, by measuring the impedance of the synthesized PEDOT scaffolds, the growth of the cells could be monitored.
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影响因子：
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