Injectable in situ forming biodegradable chitosan-hyaluronic acid based hydrogels for cartilage tissue engineering.
Injectable in situ forming biodegradable chitosan-hyaluronic acid based hydrogels for cartilage tissue engineering.
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DOI：
10.1016/j.biomaterials.2008.12.080
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发表时间：
2009-05
期刊：
影响因子：
14
通讯作者：
Marra, Kacey G.
中科院分区：
文献类型：
--
作者：
Tan, Huaping;Chu, Constance R.;Payne, Karin A.;Marra, Kacey G.
可注射、可生物降解的支架是组织工程和药物输送的重要生物材料。源自天然多糖的水凝胶是理想的支架，因为它们类似于由各种糖胺聚糖（GAG）组成的组织的细胞外基质。在这里，我们报告了一种新型的生物相容性和可生物降解的复合水凝胶，其由水溶性壳聚糖和氧化透明质酸混合而成，无需添加化学交联剂。凝胶化归因于多糖衍生物的氨基和醛基之间的席夫碱反应。在目前的工作中，合成了N-琥珀酰壳聚糖（S-CS）和醛透明质酸（A-HA）来制备复合水凝胶。通过 FTIR 光谱对多糖衍生物和复合水凝胶进行了表征。考察了S-CS和A-HA的比例对复合水凝胶的凝胶时间、微观结构、表面形貌、平衡溶胀、压缩模量和体外降解的影响。通过体外将牛关节软骨细胞封装在复合水凝胶基质内，证明了复合水凝胶作为可注射支架的潜力。结果表明，复合水凝胶支持细胞存活，并且细胞保留了软骨细胞形态。这些特性为在组织工程应用中使用可注射复合水凝胶提供了潜在的机会。
Injectable, biodegradable scaffolds are important biomaterials for tissue engineering and drug delivery. Hydrogels derived from natural polysaccharides are ideal scaffolds as they resemble the extracellular matrices of tissues comprised of various glycosaminoglycans (GAG). Here, we report a new class of biocompatible and biodegradable composite hydrogels derived from water-soluble chitosan and oxidized hyaluronic acid upon mixing, without the addition of a chemical crosslinking agent. The gelation is attributed to the Schiff-base reaction between amino and aldehyde groups of polysaccharide derivatives. In the current work, N-succinyl-chitosan (S-CS) and aldehyde hyaluronic acid (A-HA) were synthesized for preparation of the composite hydrogels. The polysaccharide derivatives and composite hydrogels were characterized by FTIR spectroscopy. The effect of the ratio of S-CS and A-HA on the gelation time, microstructure, surface morphology, equilibrium swelling, compressive modulus, and in vitro degradation of composite hydrogels was examined. The potential of the composite hydrogel as an injectable scaffold was demonstrated by encapsulation of bovine articular chondrocytes within the composite hydrogel matrix in vitro. The results demonstrated that the composite hydrogel supported cell survival and the cells retained chondrocytic morphology. These characteristics provide a potential opportunity to use the injectable, composite hydrogels in tissue engineering applications.
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