SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis.
SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis.
复制标题
SUMO E3 连接酶 AtMMS21 是拟南芥正常减数分裂和配子体发育所必需的
DOI：
10.1186/1471-2229-14-153
复制
发表时间：
2014-06-03
影响因子：
5.3
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Liu M;Shi S;Zhang S;Xu P;Lai J;Liu Y;Yuan D;Wang Y;Du J;Yang C
背景：MMS 21是真核生物中保守的SUMO E3连接酶，此前已被证明是DNA修复和维持染色体完整性所需的。拟南芥MMS 21的损失导致有缺陷的分生组织和矮化phenotypes.Results：在这里，我们显示了AtMMS 21在配子体发育过程中的作用。AtMMS 21缺陷型植株为半不育，成熟角果较短，种子败育。mms 21 -1突变体表现出花粉数量、生活力、萌发和异常花粉管生长的减少。胚囊发育也受到损害的突变体。在减数分裂过程中，观察到染色体错误分离和断裂，减数分裂产物通常是二分体或不规则的四分体。与染色体维持和行为相关的减数分裂基因的几个转录本被改变。此外，mms 21 -1花粉粒中SUMO蛋白缀合物的积累与野生型花粉粒中的SUMO蛋白缀合物不同。结论：这些结果表明AtMMS 21介导的SUMO化可能稳定减数分裂蛋白的表达和积累，并影响配子体发育。
Background:MMS21 is a SUMO E3 ligase that is conserved in eukaryotes, and has previously been shown to be required for DNA repair and maintenance of chromosome integrity. Loss of the Arabidopsis MMS21 causes defective meristems and dwarf phenotypes.Results:Here, we show a role for AtMMS21 during gametophyte development. AtMMS21 deficient plants are semisterile with shorter mature siliques and abortive seeds. The mms21-1 mutant shows reduced pollen number, and viability, and germination and abnormal pollen tube growth. Embryo sac development is also compromised in the mutant. During meiosis, chromosome mis-segregation and fragmentation is observed, and the products of meiosis are frequently dyads or irregular tetrads. Several transcripts for meiotic genes related to chromosome maintenance and behavior are altered. Moreover, accumulation of SUMO-protein conjugates in the mms21-1 pollen grains is distinct from that in wild-type.Conclusions:Thus, these results suggest that AtMMS21 mediated SUMOylation may stabilize the expression and accumulation of meiotic proteins and affect gametophyte development.
登录
查看更多内容
拟南芥 SUMO E3 连接酶 AtMMS21 是 NSE2/MMS21 的同源物，调节根部细胞增殖
影响因子：
7.2
作者：
Huang, Lixia;Yang, Songguang;Yang, Chengwei
通讯作者：
Yang, Chengwei
影响因子：
11.6
作者：
Ishida, Takashi;Fujiwara, Sumire;Sugimoto, Keiko
通讯作者：
Sugimoto, Keiko
影响因子：
7.2
作者：
Klimyuk, VI;Jones, JDG
通讯作者：
Jones, JDG
影响因子：
7.2
作者：
Jiang, Ling;Xia, Ming;Makaroff, Christopher A.
通讯作者：
Makaroff, Christopher A.
SUMO E3 连接酶 SIZ1 突变破坏拟南芥成熟雌配子体
影响因子：
3.7
作者：
Ling Y;Zhang C;Chen T;Hao H;Liu P;Bressan RA;Hasegawa PM;Jin JB;Lin J
通讯作者：
Lin J