Targeted proteomics of appendicular skeletal muscle mass and handgrip strength in black South Africans: a cross-sectional study.
Targeted proteomics of appendicular skeletal muscle mass and handgrip strength in black South Africans: a cross-sectional study.
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DOI：
10.1038/s41598-022-13548-9
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发表时间：
2022-06-09
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
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虽然阑尾骨骼肌质量（ASM）和握力（HGS）是肌肉减少症的关键组成部分，但其潜在的生物学机制尚不清楚。我们的目的是调查南非中年黑人中循环生物标志物与ASM和HGS的关系。这项研究包括934名来自中年索韦托队列的南非黑人（469名男性和465名女性，年龄在41-72岁之间）。采用线性回归模型检验182种生物标志物（用接近延伸法测量）与双能x射线吸收仪测量的ASM和测力仪测量的HGS之间的关系。混杂因素包括年龄、身高、性别、吸烟、饮酒、食物不安全、身体活动、内脏脂肪组织、艾滋病毒和更年期状况。显示性别相互作用的回归模型按性别分层。采用Benjamini-Hochberg错误发现率（FDR）进行多重检验控制，报告FDR调整后的P值。在总样本中，10个生物标志物与高ASM相关，29个与低ASM相关（P < 0.05）。在这39个生物标志物中，8个也与较低的HGS相关（P < 0.05）。在整个样本中，MMP-7仅与较低的HGS相关（P = 0.011）。ASM的52个生物标志物存在性别相互作用（P < 0.05）， HGS的6个存在性别相互作用。对于男性，LEP、MEPE和SCF与较高的ASM相关（分别为P < 0.001, = 0.004, = 0.006）， MEPE和SCF也与较高的HGS相关（分别为P = 0.001, 0.012）。同样在男性中，37种生物标志物与较低的ASM相关（P < 0.05），但这些标志物与较低的HGS无关。此外，在男性中，DLK-1和肌红蛋白仅与较高的HGS相关（P分别= 0.004和0.006），而GAL-9仅与较低的HGS相关（P = 0.005）。对于女性，LEP、CD163、IL6、TNF-R1和TNF-R2与较高的ASM相关（P < 0.001, = 0.014, = 0.027, = 0.014, = 0.048），而IGFBP-2、CTRC和RAGE与较低的ASM相关（P = 0.043, 0.001, 0.014）。没有生物标志物与女性HGS相关。总之，大多数生物标志物与ASM相关，而与HGS无关，并且在中年南非黑人中，与ASM和HGS相关的生物标志物表现出性别特异性。蛋白质组学研究应分别检查ASM和HGS。未来的研究还应考虑肌少症病理生理中的两性二态性，以发展性别特异性的治疗和诊断方法。
Although appendicular skeletal muscle mass (ASM) and handgrip strength (HGS) are key components of sarcopenia, their underlying biological mechanisms remain poorly understood. We aimed to investigate associations of circulating biomarkers with ASM and HGS in middle-aged black South Africans. This study consisted of 934 black South Africans (469 men and 465 women, aged 41–72 years) from the Middle-aged Soweto cohort. Linear regression models were used to examine relationships between 182 biomarkers (measured with proximity extension assay) and dual-energy X-ray absorptiometry-measured ASM and dynamometer-measured HGS. Age, height, sex, smoking, alcohol, food insecurity, physical activity, visceral adipose tissue, HIV and menopausal status were included as confounders. Regression models showing sex-interactions were stratified by sex. The Benjamini–Hochberg false discovery rate (FDR) was used to control for multiple testing, and FDR-adjusted P values were reported. In the total sample, 10 biomarkers were associated with higher ASM and 29 with lower ASM (P < 0.05). Out of these 39 biomarkers, 8 were also associated with lower HGS (P < 0.05). MMP-7 was associated with lower HGS only (P = 0.011) in the total sample. Sex-interactions (P < 0.05) were identified for 52 biomarkers for ASM, and 6 for HGS. For men, LEP, MEPE and SCF were associated with higher ASM (P < 0.001, = 0.004, = 0.006, respectively), and MEPE and SCF were also associated with higher HGS (P = 0.001, 0.012, respectively). Also in men, 37 biomarkers were associated with lower ASM (P < 0.05), with none of these being associated with lower HGS. Furthermore, DLK-1 and MYOGLOBIN were associated with higher HGS only (P = 0.004, 0.006, respectively), while GAL-9 was associated with lower HGS only (P = 0.005), among men. For women, LEP, CD163, IL6, TNF-R1 and TNF-R2 were associated with higher ASM (P < 0.001, = 0.014, = 0.027, = 0.014, = 0.048, respectively), while IGFBP-2, CTRC and RAGE were associated with lower ASM (P = 0.043, 0.001, 0.014, respectively). No biomarker was associated with HGS in women. In conclusion, most biomarkers were associated with ASM and not HGS, and the associations of biomarkers with ASM and HGS displayed sex-specificity in middle-aged black South Africans. Proteomic studies should examine ASM and HGS individually. Future research should also consider sexual dimorphism in the pathophysiology of sarcopenia for development of sex-specific treatment and diagnostic methods.
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DOI：
10.1002/jcsm.12733
发表时间：
2021-08
期刊：
Journal of cachexia, sarcopenia and muscle
影响因子：
--
作者：
Huemer MT;Bauer A;Petrera A;Scholz M;Hauck SM;Drey M;Peters A;Thorand B
通讯作者：
Thorand B
影响因子：
16.6
作者：
Sartori R;Romanello V;Sandri M
通讯作者：
Sandri M
影响因子：
1.4
作者：
Dimitrova, Ivelina;Tacheva, Tanya;Vlaykova, Tatyana
通讯作者：
Vlaykova, Tatyana
影响因子：
4.5
作者：
McLeod M;Breen L;Hamilton DL;Philp A
通讯作者：
Philp A
DOI：
10.3109/13697137.2011.650656
发表时间：
2012-04
期刊：
Climacteric : the journal of the International Menopause Society
影响因子：
--
作者：
Harlow SD;Gass M;Hall JE;Lobo R;Maki P;Rebar RW;Sherman S;Sluss PM;de Villiers TJ;STRAW+10 Collaborative Group
通讯作者：
STRAW+10 Collaborative Group