Controllable synthesis of NaYF(4) : Yb,Er upconversion nanophosphors and their application to in vivo imaging of Caenorhabditis elegans.
Controllable synthesis of NaYF(4) : Yb,Er upconversion nanophosphors and their application to in vivo imaging of Caenorhabditis elegans.
复制标题
DOI：
10.1039/c0jm02854a
复制
发表时间：
2011-02-28
影响因子：
--
通讯作者：
Xu S
中科院分区：
文献类型：
--
作者：
Chen J;Guo C;Wang M;Huang L;Wang L;Mi C;Li J;Fang X;Mao C;Xu S
β-NaYF4：Yb，Er上转换纳米粒子（UCNPs）在近红外（NIR）光激发下能发出明亮的绿色荧光，对人体安全，并能深入组织。近年来，UCNPs在生物标记和成像方面的应用受到了广泛关注。本文以室温离子液体为反应物、助溶剂和模板剂，采用一步绿色溶剂热法合成了平均粒径为35 nm、球形均匀、表面氨基修饰的β-NaYF 4：Yb，Er纳米碳纳米管。将所制备的UCNPs导入秀丽隐杆线虫（C. habditiselegans）中。elegans）以实现成功的体内成像。我们发现，较长的孵育时间、较高的UCNP浓度和较小的UCNP尺寸可以使C.更明亮，沿着沿着更连续。蠕虫对具有相似大小和形状的用不同封端配体封端的UCNP的摄取没有明显的选择性。下一代蠕虫在激发下不显示荧光。此外，通过研究UCNP存在下蠕虫的存活率证明了纳米颗粒的低毒性。我们的工作展示了UCNPs在研究生物体生物学行为方面的潜在应用，并为进一步发展UCNPs在疾病检测和诊断方面的应用奠定了基础。
β-NaYF4 : Yb,Er upconversion nanoparticles (UCNPs) can emit bright green fluorescence under near-infrared (NIR) light excitation which is safe to the body and can penetrate deeply into tissues. The application of UCNPs in biolabeling and imaging has received great attention recently. In this work, β-NaYF4 : Yb,Er UCNPs with an average size of 35 nm, uniformly spherical shape, and surface modified with amino groups were synthesized by a one-step green solvothermal approach through the use of room-temperature ionic liquids as the reactant, co-solvent and template. The as-prepared UCNPs were introduced into Caenorhabditis elegans (C. elegans) to achieve successful in vivo imaging. We found that longer incubation time, higher UCNP concentration and smaller UCNP size can make the in vivo fluorescence of C. elegans much brighter and more continuous along their body. The worms have no apparent selectivity on ingestion of the UCNPs capped with different capping ligands while having similar size and shape. The next generation of worms did not show fluorescence under excitation. In addition, low toxicity of the nanoparticles was demonstrated by investigating the survival rates of the worms in the presence of the UCNPs. Our work demonstrates the potential application of the UCNPs in studying the biological behavior of organisms, and lays the foundation for further development of the UCNPs in the detection and diagnosis of diseases.
登录
查看更多内容
影响因子：
--
作者：
Lu, HC;Yi, GS;Cheng, J
通讯作者：
Cheng, J
影响因子：
46.9
作者：
Jaiswal, JK;Mattoussi, H;Simon, SM
通讯作者：
Simon, SM
影响因子：
56.9
作者：
Bruchez, M;Moronne, M;Alivisatos, AP
通讯作者：
Alivisatos, AP
影响因子：
46.9
作者：
Han, MY;Gao, XH;Nie, S
通讯作者：
Nie, S
影响因子：
7.4
作者：
Male, Keith B.;Lachance, Bernard;Luong, John H. T.
通讯作者：
Luong, John H. T.