The anatomy of transcriptionally active chromatin loops in Drosophila primary spermatocytes using super-resolution microscopy
The anatomy of transcriptionally active chromatin loops in Drosophila primary spermatocytes using super-resolution microscopy
复制标题
使用超分辨率显微镜对果蝇原代精母细胞中转录活性染色质环进行解剖
DOI：
10.1101/2022.07.27.500934
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Ball M
中科院分区：
文献类型：
--
作者：
Ball M
虽然基因转录的生物化学已经得到了很好的研究，但我们对这一过程如何在完整的细胞核内以3D方式组织的理解还不太清楚。在这里，我们调查的结构，积极转录的染色质和建筑的相互作用与积极的RNA聚合酶。为了进行这项分析，我们使用超分辨率显微镜对果蝇Y环进行成像，这些Y环代表了巨大的、几兆字节长的单个转录单位。Y环为转录活性染色质提供了一个特别适合的模型系统。我们发现，尽管这些转录的环是解密集的，但它们并没有组织成延伸的10 nm纤维，而是主要由核小体簇的链组成。每个团簇的平均宽度约为50nm。我们发现，活跃的RNA聚合酶的焦点通常位于核小体簇外围的主纤维轴之外。RNA聚合酶和新生转录物的焦点分布在Y环周围，而不是聚集在单个转录工厂中。然而，由于RNA聚合酶灶比核小体簇普遍得多，因此这种活性染色质组织成核小体簇链不太可能由转录Y环的聚合酶的活性决定。这些结果为理解染色质与基因转录过程的拓扑关系提供了基础。
While the biochemistry of gene transcription has been well studied, our understanding of how this process is organised in 3D within the intact nucleus is less well understood. Here we investigate the structure of actively transcribed chromatin and the architecture of its interaction with active RNA polymerase. For this analysis, we have used super-resolution microscopy to image theDrosophila melanogasterY loops which represent huge, several megabases long, single transcription units. The Y loops provide a particularly amenable model system for transcriptionally active chromatin. We find that, although these transcribed loops are decondensed they are not organised as extended 10nm fibres, but rather they largely consist of chains of nucleosome clusters. The average width of each cluster is around 50nm. We find that foci of active RNA polymerase are generally located off the main fibre axis on the periphery of the nucleosome clusters. Foci of RNA polymerase and nascent transcripts are distributed around the Y loops rather than being clustered in individual transcription factories. However, as the RNA polymerase foci are considerably less prevalent than the nucleosome clusters, the organisation of this active chromatin into chains of nucleosome clusters is unlikely to be determined by the activity of the polymerases transcribing the Y loops. These results provide a foundation for understanding the topological relationship between chromatin and the process of gene transcription.
登录
查看更多内容
DOI：
10.1109/34.1000236
发表时间：
2002-05-01
影响因子：
23.6
作者：
Comaniciu, D;Meer, P
通讯作者：
Meer, P
DOI：
10.1083/jcb.200011069
发表时间：
2001-07-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Müller WG;Walker D;Hager GL;McNally JG
通讯作者：
McNally JG
影响因子：
64.5
作者：
Rao SS;Huntley MH;Durand NC;Stamenova EK;Bochkov ID;Robinson JT;Sanborn AL;Machol I;Omer AD;Lander ES;Aiden EL
通讯作者：
Aiden EL
灯刷染色体
DOI：
10.1007/978-3-642-82792-1
发表时间：
1986
期刊：
Molecular biology, biochemistry, and biophysics
影响因子：
--
作者：
Professor Dr. Harold Garnet Callan
通讯作者：
Professor Dr. Harold Garnet Callan
果蝇精子发生的减数分裂前、减数分裂和减数分裂后早期的染色质和微管组织。
影响因子：
4
作者：
G. Cenci;S. Bonaccorsi;Claudio Pisano;F. Vernì;M. Gatti
通讯作者：
M. Gatti