Crucial role of perilipin-3 (TIP47) in formation of lipid droplets and PGE2 production in HL-60-derived neutrophils.
Crucial role of perilipin-3 (TIP47) in formation of lipid droplets and PGE2 production in HL-60-derived neutrophils.
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DOI：
10.1371/journal.pone.0071542
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Itabe H
中科院分区：
文献类型：
--
作者：
Nose F;Yamaguchi T;Kato R;Aiuchi T;Obama T;Hara S;Yamamoto M;Itabe H
细胞溶质脂滴（LD）现在被认为是多功能细胞器，在各种炎症条件下在白细胞中积聚。然而，人们对中性粒细胞中 LD 的特征知之甚少。在这项研究中，我们发现 perilipin-3（PLIN3；以前称为 TIP47）参与 LD 形成和 HL-60 衍生的中性粒细胞的炎症反应。 HL-60 是一种早幼粒细胞系，通过全反式视黄酸处理分化为中性粒细胞。分化后，用牙龈卟啉单胞菌脂多糖（P.g-LPS）（成人牙周炎的主要病原体）刺激细胞。当用 P.g-LPS 刺激 HL-60 衍生的中性粒细胞时，LD 的数量和大小均增加。在分化的细胞中，PLIN3被诱导，而PLIN1、PLIN2和PLIN5未检测到。 P.g-LPS 处理细胞后，PGE2 产量和 PLIN3 蛋白水平以剂量依赖性方式增加。当用 siRNA 处理下调 PLIN3 时，LD 基本消失，细胞培养基中分泌的 PGE2 水平下降 65%。此外，抑制 PLIN3 也会抑制 PGE2 产生酶；即微粒体 PGE 合酶-1、-2 和环氧合酶-2。这些发现表明，PLIN3 在 HL-60 衍生的中性粒细胞的 LD 生物发生中具有关键作用，并且 PLIN3 与 PGE2 的合成和分泌相关。
Cytosolic lipid droplets (LDs), which are now recognized as multifunctional organelles, accumulate in leukocytes under various inflammatory conditions. However, little is known about the characteristic features of LDs in neutrophils. In this study, we show that perilipin-3 (PLIN3; formerly called TIP47) is involved in LD formation and the inflammatory response in HL-60-derived neutrophils. HL-60, a promyelocytic cell line, was differentiated into neutrophils via treatment with all-trans retinoic acid. After differentiation, cells were stimulated with Porphyromonas gingivalis lipopolysaccharide (P.g-LPS), a major pathogen in adult periodontitis. When HL-60-derived neutrophils were stimulated with P.g-LPS, LDs increased in both number and size. In the differentiated cells, PLIN3 was induced while PLIN1, PLIN2 and PLIN5 were not detected. PGE2 production and the PLIN3 protein level were increased by the P.g-LPS treatment of the cells in a dose-dependent manner. When PLIN3 was down-regulated with siRNA treatment, LDs essentially disappeared and the level of PGE2 secreted in the cell culture medium decreased by 65%. In addition, the suppression of PLIN3 repressed the PGE2 producing enzymes; i.e., microsomal PGE synthase-1, -2 and cyclooxygenase-2. These findings indicate that PLIN3 has a pivotal role in LD-biogenesis in HL-60-derived neutrophils, and that PLIN3 is associated with the synthesis and secretion of PGE2.
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影响因子：
6.5
作者：
Kimmel AR;Brasaemle DL;McAndrews-Hill M;Sztalryd C;Londos C
通讯作者：
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作者：
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DOI：
10.1073/pnas.77.5.2936
发表时间：
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期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/j.bbalip.2009.04.002
发表时间：
2009-06
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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