Structural analysis and anti-complement activity of polysaccharides from Kjellmaniella crsaaifolia.
Structural analysis and anti-complement activity of polysaccharides from Kjellmaniella crsaaifolia.
复制标题
凯氏藻多糖的结构分析及抗补体活性
DOI：
10.3390/md13031360
复制
发表时间：
2015-03-16
期刊：
影响因子：
5.4
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Zhang W;Jin W;Sun D;Zhao L;Wang J;Duan D;Zhang Q
用稀盐酸和水分别从青海海棠中提取了两种多糖，分别命名为KCA和KCW。组成分析表明，这些多糖主要由岩藻糖组成，其次是半乳糖、甘露糖和葡萄糖醛酸。经降解和部分脱硫后，进行电喷雾电离质谱仪(ESI-MS)分析，结果表明该多糖由硫酸化岩藻低聚糖、硫酸岩藻低聚半乳糖和单硫酸/多硫酸岩藻低聚糖的甲基糖苷组成。用电喷雾电离碰撞诱导解离串联质谱仪(ESI-CID-MS2和ESI-CID-MS3)进一步表征了寡聚片段的结构。此外，还测定了KCA和KCW对经典和替代补体途径的溶血活性。KCA的活性与KCW相似，说明提取方法不影响KCA的活性。此外，降解多糖(DKCA和DKCW)的活性水平低于粗多糖(KCA和KCW)，说明分子量对活性有影响。此外，脱硫组分(DS-DKCA和DS-DKCW)显示较低的活性或没有活性，这证实了硫酸盐对活性的重要性。综上所述，粗多糖可能是治疗补体途径相关疾病的良好候选物质。
Two polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water, respectively. Composition analysis showed that these polysaccharides predominantly consisted of fucose, with galactose, mannose and glucuronic acid as minor components. After degradation and partial desulfation, electrospray ionization mass spectrometry (ESI-MS) was performed, which showed that the polysaccharides consisted of sulfated fucooligosaccharides, sulfated galactofucooligosaccharides and methyl glycosides of mono-sulfated/multi-sulfated fucooligosaccharides. The structures of the oligomeric fragments were further characterized by electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS2 and ESI-CID-MS3). Moreover, the activity of KCA and KCW against the hemolytic activity of both the classical and alternative complement pathways was determined. The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity. In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity. Moreover, the desulfated fractions (ds-DKCA and ds-DKCW) showed less or no activity, which confirmed that sulfate was important for activity. In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway.
登录
查看更多内容
影响因子：
14
作者：
Blondin, C;Chaubet, F;Jozefonvicz, J
通讯作者：
Jozefonvicz, J
影响因子：
2.9
作者：
BITTER, T;MUIR, HM
通讯作者：
MUIR, HM
影响因子：
3.1
作者：
Jin, Weihua;Zhang, Wenjing;Zhang, Quanbin
通讯作者：
Zhang, Quanbin
影响因子：
11.2
作者：
Chen, Shiguo;Xue, Changhu;Chai, Wengang
通讯作者：
Chai, Wengang
影响因子：
3.1
作者：
Jin, Weihua;Guo, Zhimou;Zhang, Quanbin
通讯作者：
Zhang, Quanbin