Gustatory solitary tract development: a role for neuropilins.
Gustatory solitary tract development: a role for neuropilins.
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DOI：
10.1016/j.neuroscience.2013.07.068
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发表时间：
2013-11-12
期刊：
影响因子：
3.3
通讯作者：
Bradley RM
中科院分区：
文献类型：
--
作者：
Corson SL;Kim M;Mistretta CM;Bradley RM
孤立束吻侧核（rNST）通过颅神经VII和颅神经IX接收来自舌和腭味蕾细胞的口感信息。这些神经进入脑干，形成孤立束（ST），并与rNST中的神经元突触，然后将传入的感觉信息传递到其他大脑区域，以处理外部味觉刺激。指导或调节ST发展轨迹的因素在很大程度上是未知的。我们使用DiI鉴定胚胎大鼠从胚胎14天至18天（E14-E18）的膝状神经节细胞中产生的ST投射。鉴定ST纤维后，对轴突引导分子neuropilin-1 （Npn-1）和neuropilin-2 （Npn-2）及其结合伙伴semaphorin-3A （Sema-3A）和semaphorin-3F （Sema-3F）进行免疫标记和蛋白表达分析。结果详细说明了ST投射到味觉脑干的形成及其与rNST神经元发育的关系。早在E14时脑干中就存在dii标记的ST纤维。Npn-1在E14时在ST和三叉道中表达，但在E18时蛋白水平下降。Npn-1结合伙伴Sema-3A的表达量从E14到E18增加。在E14时，Npn-2在ST中表达，此外，在脑干内径向取向的簇状结构中表达。Npn-2的表达水平在E18期间也有所下降，而其结合伙伴Sema-3F的表达水平在此期间有所上升。首次确定了ST发育的时间过程和特定的分子成分。这些结果表明，轴突引导分子中的神经肽和信号蛋白家族是ST形成的潜在分子参与者。
The rostral nucleus of the solitary tract (rNST) receives orosensory information from taste bud cells in the tongue and palate via cranial nerves VII and IX. These nerves enter the brainstem, form the solitary tract (ST) and synapse with neurons in the rNST, which then relay incoming sensory information to other brain areas to process external gustatory stimuli. Factors that direct or regulate the trajectory of the developing ST are largely unknown. We used DiI to identify ST projections originating from cells in the geniculate ganglia of embryonic rats from embryonic day 14 through 18 (E14-E18). After identifying the ST fibers, immunolabeling for and protein expression analysis of the axon guidance molecules neuropilin-1 (Npn-1) and neuropilin-2 (Npn-2) and their binding partners, semaphorin-3A (Sema-3A) and semaphorin-3F (Sema-3F) were performed. The results detail the formation of ST projections into the gustatory brainstem and their relationship to developing rNST neurons. DiI-labeled ST fibers were present in the brainstem as early as E14. Npn-1 was expressed in the ST and in the trigeminal tract at E14, but levels of the protein declined through E18. The expression levels of the binding partner of Npn-1, Sema-3A, increased from E14 to E18. Npn-2 was expressed in the ST and, additionally, in radially oriented, tuft-like structures within the brainstem at E14. Expression levels of Npn-2 also declined through E18, in contrast to the expression levels of its binding partner, Sema-3F, which increased during this time period. For the first time, the time course and particular molecular components involved in development of the ST have been identified. These results indicate that the neuropilin and semaphorin families of axon guidance molecules are potential molecular participants in ST formation.
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DOI：
10.1083/jcb.200903137
发表时间：
2010-01-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Xu Y;Yuan L;Mak J;Pardanaud L;Caunt M;Kasman I;Larrivée B;Del Toro R;Suchting S;Medvinsky A;Silva J;Yang J;Thomas JL;Koch AW;Alitalo K;Eichmann A;Bagri A
通讯作者：
Bagri A
影响因子：
2.5
作者：
Mangold, Jamie E.;Hill, David L.
通讯作者：
Hill, David L.
影响因子：
2.7
作者：
Patel, Ami V.;Krimm, Robin F.
通讯作者：
Krimm, Robin F.
影响因子：
2.9
作者：
Suwabe T;Mistretta CM;Bradley RM
通讯作者：
Bradley RM
影响因子：
5.3
作者：
Mangold, Jamie E.;Hill, David L.
通讯作者：
Hill, David L.