SPL36 Encodes a Receptor-like Protein Kinase that Regulates Programmed Cell Death and Defense Responses in Rice.
SPL36 Encodes a Receptor-like Protein Kinase that Regulates Programmed Cell Death and Defense Responses in Rice.
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DOI：
10.1186/s12284-021-00475-y
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发表时间：
2021-04-07
期刊：
影响因子：
--
通讯作者：
Yuexing WANG
中科院分区：
文献类型：
--
作者：
Yuchun RAO;Ran JIAO;Sheng WANG;Xianmei WU;Hanfei YE;Chenyang PAN;Sanfeng LI;Dedong X;Weiyong ZHOU;Gaoxing DAI;Juan HU;Deyong REN;Yuexing WANG
在没有生物或非生物胁迫的情况下，病变模拟突变体自发地产生病斑。对拟病斑突变体的分析有助于阐明植物细胞程序性死亡和防御反应的机制。在这里，我们分离和表征的水稻（Oryza sativa）斑点叶36（spl 36）突变体，这是从甲基磺酸乙酯诱变的粳稻品种云岛人口。spl 36表现出自发细胞死亡并增强了对水稻细菌病原体的抗性。基因表达分析表明，spl 36通过上调防御相关基因的表达在疾病反应中发挥作用。生理生化试验表明，突变体spl 36的细胞死亡率高于野生型，植株生长发育受到影响。我们通过图位克隆分离了SPL 36。在spl 36中检测到单个碱基取代，这导致SPL 36中半胱氨酸被精氨酸取代。预测SPL 36编码一个含有富含亮氨酸结构域的受体样蛋白激酶，该结构域可能参与水稻的胁迫反应。spl 36比野生型对盐胁迫更敏感，表明SPL 36也负调节盐胁迫响应。这些发现表明，SPL 36通过影响防御和胁迫相关基因的表达来调节水稻的抗病反应。在线版本包含补充材料，可通过10.1186/s12284-021-00475-y获得。
Lesion mimic mutants spontaneously produce disease spots in the absence of biotic or abiotic stresses. Analyzing lesion mimic mutants’ sheds light on the mechanisms underlying programmed cell death and defense-related responses in plants. Here, we isolated and characterized the rice (Oryza sativa) spotted leaf 36 (spl36) mutant, which was identified from an ethyl methanesulfonate-mutagenized japonica cultivar Yundao population. spl36 displayed spontaneous cell death and enhanced resistance to rice bacterial pathogens. Gene expression analysis suggested that spl36 functions in the disease response by upregulating the expression of defense-related genes. Physiological and biochemical experiments indicated that more cell death occurred in spl36 than the wild type and that plant growth and development were affected in this mutant. We isolated SPL36 by map-based cloning. A single base substitution was detected in spl36, which results in a cysteine-to-arginine substitution in SPL36. SPL36 is predicted to encode a receptor-like protein kinase containing leucine-rich domains that may be involved in stress responses in rice. spl36 was more sensitive to salt stress than the wild type, suggesting that SPL36 also negatively regulates the salt-stress response. These findings suggest that SPL36 regulates the disease resistance response in rice by affecting the expression of defense- and stress-related genes. The online version contains supplementary material available at 10.1186/s12284-021-00475-y.
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DOI：
10.1016/s0167-4781(03)00120-9
发表时间：
2004-02-20
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Jung, EH;Jung, HW;Hwang, BK
通讯作者：
Hwang, BK
影响因子：
7.4
作者：
Hong, SW;Jon, JH;Nam, HG
通讯作者：
Nam, HG
影响因子：
4.9
作者：
Campbell, MA;Ronald, PC
通讯作者：
Ronald, PC
影响因子：
11.6
作者：
Gómez-Gómez, L;Bauer, Z;Boller, T
通讯作者：
Boller, T
影响因子：
5.6
作者：
Petrov V;Hille J;Mueller-Roeber B;Gechev TS
通讯作者：
Gechev TS