Stem cells commit to differentiation following multiple induction events in the Drosophila testis
Stem cells commit to differentiation following multiple induction events in the Drosophila testis
复制标题
果蝇睾丸中的多次诱导事件后干细胞致力于分化
DOI：
10.1101/2021.05.20.444930
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yuen A
中科院分区：
文献类型：
--
作者：
Yuen A
如何以及何时潜能在分化干细胞子细胞时受到限制还知之甚少。虽然认为来自小生境的信号是防止分化所必需的，但另一个模型提出干细胞可以在多种状态之间可逆地转换，其中一些状态是启动的，但不是致力于分化。在果蝇睾丸中，体细胞囊肿干细胞（CySC）产生囊细胞，囊细胞包裹生殖细胞以支持其发育。我们发现，如果PI 3 K/Tor通路的活性被抑制，CySC的维持独立于小生境自我更新信号。相反，PI 3 K/Tor不足以单独驱动分化，这表明它起着许可细胞分化的作用。事实上，我们发现生殖系是响应PI 3 K/Tor升高的CySC分化所必需的，这表明最终分化涉及几个步骤和细胞间通讯。我们认为CySC子细胞是可塑的，它们的命运取决于邻近生殖细胞的可用性，PI 3 K/Tor的作用是诱导CySC子细胞的启动状态，使其与生殖细胞协调分化。
How and when potential becomes restricted in differentiating stem cell daughters is poorly understood. While it is thought that signals from the niche are actively required to prevent differentiation, another model proposes that stem cells can reversibly transit between multiple states, some of which are primed, but not committed, to differentiate. In the Drosophila testis, somatic cyst stem cells (CySCs) generate cyst cells, which encapsulate the germline to support its development. We find that CySCs are maintained independently of niche self-renewal signals if activity of the PI3K/Tor pathway is inhibited. Conversely, PI3K/Tor is not sufficient alone to drive differentiation, suggesting that it acts to license cells for differentiation. Indeed, we find that the germline is required for differentiation of CySCs in response to PI3K/Tor elevation, indicating that final commitment to differentiation involves several steps and intercellular communication. We propose that CySC daughter cells are plastic, that their fate depends on the availability of neighbouring germ cells, and that PI3K/Tor acts to induce a primed state for CySC daughters to enable coordinated differentiation with the germline.
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