Insight of low-abundance proteins in rice leaves under Cd stress using combinatorial peptide ligand library technology
Insight of low-abundance proteins in rice leaves under Cd stress using combinatorial peptide ligand library technology
复制标题
利用组合肽配体库技术洞察镉胁迫下水稻叶片中的低丰度蛋白质
DOI：
10.1007/s00216-020-02760-z
复制
发表时间：
2020-06
影响因子：
4.3
通讯作者：
Mingxue Chen
中科院分区：
文献类型：
--
作者：
Xiaoyan Lin;Shuangshuang Chai;Siqi Huang;Renxiang Mou;Zhaoyun Cao;Zhenzhen Cao;Mingxue Chen
低丰度蛋白在植物生物过程的相互作用、调节和代谢中起着非常重要的作用。组合肽配体库（CPLL）可以解决高丰度蛋白质（HAP）掩蔽LAPs的问题，并完美地扩大蛋白质浓度的动态范围，被认为是植物蛋白质组学研究最先进的方法之一。本研究首次建立了水稻叶片蛋白质的CPLL方法，共鉴定出1056个蛋白质，其中624个（59.1%）为新检测到的LAPs。在此基础上，检测了水稻对Cd胁迫的响应，并利用生物信息学方法分析了Cd胁迫前后水稻LAPs的差异及差异表达蛋白的生物学意义。这一研究也为更好地理解水稻适应镉胁迫的复杂机制做出了重要贡献。
Low-abundance proteins (LAPs) play a very important role in interaction, regulation, and metabolism of plant biological processes. A combinatorial peptide ligand library (CPLL) can solve the problem of high-abundance proteins (HAPs) masking LAPs and enlarging the dynamic range of protein concentrations perfectly and be considered as one of the most advanced approaches for plant proteomics research. In this paper, a proper CPLL method to rice leaf proteins was established for the first time and 1056 proteins were identified in rice leaf extracts, and 624 (59.1%) LAPs were newly detected after CPLL. Based on this technology, we detected the response of rice to Cd stress and analyzed the differential LAPs and the biological significance of misexpressed proteins before and after Cd stress by bioinformatics analysis. An important contribution has also been made to a better understanding of the complex mechanisms by which rice adapts to Cd stress.Graphical abstract
登录
查看更多内容
影响因子：
5.5
作者：
Leimkühler S;Bühning M;Beilschmidt L
通讯作者：
Beilschmidt L
影响因子：
4.1
作者：
Guerrier, L.;Claverol, S.;Housset, C.
通讯作者：
Housset, C.
鉴定含有异丙醇胺接头的外消旋和手性咔唑衍生物作为抗植物病原细菌的潜在替代物：体外和体内测定以及定量蛋白质组学
影响因子：
6.1
作者：
Zhao Yong Liang;Huang Xing;Liu Li Wei;Wang Pei Yi;Long Qing Su;Tao Qing Qing;Li Zhong;Yang Song
通讯作者：
Yang Song
影响因子：
4.4
作者：
Castagna, A;Cecconi, D;Rigetti, PG
通讯作者：
Rigetti, PG
影响因子：
6.1
作者：
Cremer, Julia E.;Bean, Scott R.;Godwin, Ian D.
通讯作者：
Godwin, Ian D.