LMPA Regulates Lesion Mimic Leaf and Panicle Development Through ROS-Induced PCD in Rice.
LMPA Regulates Lesion Mimic Leaf and Panicle Development Through ROS-Induced PCD in Rice.
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LMPA 通过 ROS 诱导的水稻 PCD 调节病斑模拟叶和穗发育
DOI：
10.3389/fpls.2022.875038
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
叶片是水稻重要的营养器官，穗是水稻重要的产量器官。虽然已经鉴定了几个控制病斑模拟叶和穗败育的基因，但少数研究报道了单个基因参与这两个性状的产生。在本研究中，我们鉴定了一个穗败育突变体，病叶和穗顶端败育（lmpa），它表现在叶片上的病变，并导致顶端小穗退化。分子克隆显示，LMPA编码一个质子泵ATP酶蛋白，定位于质膜，在叶片和圆锥花序中高度表达。启动子活性分析表明，在lmpa启动子中插入一个片段引起转录水平下降。细胞和组织化学分析表明，lmpa细胞内活性氧积累，细胞死亡。此外，生理实验表明lmpa对高温和盐胁迫条件更敏感。这些结果提供了一个更好的理解LMPA在穗发育和病变模拟形成通过调节活性氧稳态的作用。
Leaf and panicle are important nutrient and yield organs in rice, respectively. Although several genes controlling lesion mimic leaf and panicle abortion have been identified, a few studies have reported the involvement of a single gene in the production of both the traits. In this study, we characterized a panicle abortion mutant, lesion mimic leaf and panicle apical abortion (lmpa), which exhibits lesions on the leaf and causes degeneration of apical spikelets. Molecular cloning revealed that LMPA encodes a proton pump ATPase protein that is localized in the plasma membrane and is highly expressed in leaves and panicles. The analysis of promoter activity showed that the insertion of a fragment in the promoter of lmpa caused a decrease in the transcription level. Cellular and histochemistry analysis indicated that the ROS accumulated and cell death occurred in lmpa. Moreover, physiological experiments revealed that lmpa was more sensitive to high temperatures and salt stress conditions. These results provide a better understanding of the role of LMPA in panicle development and lesion mimic formation by regulating ROS homeostasis.
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