Boundary stiffness regulates fibroblast behavior in collagen gels.
Boundary stiffness regulates fibroblast behavior in collagen gels.
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DOI：
10.1007/s10439-009-9856-1
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发表时间：
2010-03
影响因子：
3.8
通讯作者：
Billiar, Kristen
中科院分区：
文献类型：
--
作者：
John, Jeffrey;Quinlan, Angela Throm;Silvestri, Chiara;Billiar, Kristen
最近的研究表明，细胞行为的深刻依赖于2D培养底物的硬度。这项研究的目的是开发一种方法，在不影响细胞外基质的物理化学性质的情况下，改变标准3D胶原凝胶模型中细胞的硬度。利用柔性锚(0.048-0.64N m−1)开发了一种装置，可在常用的自由和固定条件(零和无限刚性边界刚度)之间调节悬浮胶原凝胶的边界刚度。我们通过有限元分析和广泛的实验研究论证了其工作原理。在所有情况下，边界硬度对细胞行为都有很强的影响，最显著的是引起更高的基础张力和激活力(对KCl的响应)，以及在更高的边界硬度水平时更明显的胶原基质重塑。种植300万人包皮成纤维细胞的凝胶的测量等轴力范围从0.05到1mN，随着边界硬度的增加而单调增加。使用代表性体积元素分析，估计每个单元的力范围从17到100 NN。该装置为独立研究3D胶原基质中细胞机械环境的影响提供了有价值的工具。
Recent studies have illustrated the profound dependence of cellular behavior on the stiffness of 2D culture substrates. The goal of this study was to develop a method to alter the stiffness cells experience in a standard 3D collagen gel model without affecting the physiochemical properties of the extracellular matrix. A device was developed utilizing compliant anchors (0.048–0.64 N m−1) to tune the boundary stiffness of suspended collagen gels in between the commonly utilized free and fixed conditions (zero and infinite stiffness boundary stiffness). We demonstrate the principle of operation with finite element analyses and a wide range of experimental studies. In all cases, boundary stiffness has a strong influence on cell behavior, most notably eliciting higher basal tension and activated force (in response to KCl) and more pronounced remodeling of the collagen matrix at higher boundary stiffness levels. Measured equibiaxial forces for gels seeded with 3 million human foreskin fibroblasts range from 0.05 to 1 mN increasing monotonically with boundary stiffness. Estimated force per cell ranges from 17 to 100 nN utilizing representative volume element analysis. This device provides a valuable tool to independently study the effect of the mechanical environment of the cell in a 3D collagen matrix.
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影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM
DOI：
10.1115/1.429652
发表时间：
2000-06-01
影响因子：
1.7
作者：
Girton, TS;Oegema, TR;Tranquillo, RT
通讯作者：
Tranquillo, RT
影响因子：
--
作者：
Chen, Jianxin;Li, Hongxia;Wang, James H. -C.
通讯作者：
Wang, James H. -C.
影响因子：
6.5
作者：
Fluck, J;Querfeld, C;Sollberg, S
通讯作者：
Sollberg, S
影响因子：
--
作者：
Ibusuki, Shinichi;Halbesma, Gerrit J.;Gill, Thomas J.
通讯作者：
Gill, Thomas J.