In Vivo Targeted MR Imaging of Endogenous Neural Stem Cells in Ischemic Stroke.
In Vivo Targeted MR Imaging of Endogenous Neural Stem Cells in Ischemic Stroke.
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缺血性中风内源性神经干细胞的体内靶向 MR 成像
DOI：
10.3390/molecules21091143
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发表时间：
2016-08-29
期刊：
影响因子：
--
通讯作者：
Shen J
中科院分区：
文献类型：
--
作者：
Zhang F;Duan X;Lu L;Zhang X;Zhong X;Mao J;Chen M;Shen J
急性缺血性中风仍然是导致死亡和残疾的主要原因。通过激活神经干细胞(NSCs)促进内源性神经发生可能是治疗中风的一种有前途的方法。体内靶向示踪是监测卒中内源性神经干细胞动态的理想方法。此前，我们利用抗CD15抗体偶联的超顺磁性氧化铁纳米颗粒(anti-CD15-SPIOns)作为分子探针，成功地实现了正常成年小鼠脑内内源性神经干细胞的体内靶向磁共振成像。在此，我们探讨了该分子探针在体内靶向示踪缺血性卒中激活的内源性神经干细胞的性能。我们的研究表明，脑内注射抗CD15-SPION可在脑缺血后7天原位标记激活的内源性神经干细胞，在7.0T磁共振成像上可检测到激活的低信号区域扩大。阿糖胞苷治疗可抑制内源性神经干细胞的激活，其特点是磁共振成像低信号区域消失。使用抗CD15-SPION作为成像探针，可以方便地通过体内磁共振成像来监测内源性NSCs的激活的动态过程。这种靶向成像策略将对开发利用内源性神经干细胞治疗缺血性卒中的新策略大有裨益。
Acute ischemic stroke remains a leading cause of death and disability. Endogenous neurogenesis enhanced via activation of neural stem cells (NSCs) could be a promising method for stroke treatment. In vivo targeted tracking is highly desirable for monitoring the dynamics of endogenous NSCs in stroke. Previously, we have successfully realized in vivo targeted MR imaging of endogenous NSCs in normal adult mice brains by using anti-CD15 antibody-conjugated superparamagnetic iron oxide nanoparticles (anti-CD15-SPIONs) as the molecular probe. Herein, we explore the performance of this molecular probe in targeted in vivo tracking of activated endogenous NSCs in ischemic stroke. Our study showed that intraventricular injection of anti-CD15-SPIONs could label activated endogenous NSCs in situ seven days after ischemic stroke, which were detected as enlarged areas of hypo-intense signals on MR imaging at 7.0 T. The treatment of cytosine arabinosine could inhibit the activation of endogenous NSCs, which was featured by the disappearance of areas of hypo-intense signals on MR imaging. Using anti-CD15-SPIONs as imaging probes, the dynamic process of activation of endogenous NSCs could be readily monitored by in vivo MR imaging. This targeted imaging strategy would be of great benefit to develop a new therapeutic strategy utilizing endogenous NSCs for ischemic stroke.
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影响因子：
3.7
作者：
Elvira G;García I;Benito M;Gallo J;Desco M;Penadés S;Garcia-Sanz JA;Silva A
通讯作者：
Silva A
正常和受损发育大脑脑室下区内源性干/祖细胞迁移的体内 MRI
影响因子：
5.7
作者：
Yang, Jian;Liu, Jianxin;Wu, Ed X.
通讯作者：
Wu, Ed X.
DOI：
10.1073/pnas.0603512103
发表时间：
2006-08-29
影响因子：
11.1
作者：
Jin, Kunlin;Wang, Xiaomei;Greenberg, David A.
通讯作者：
Greenberg, David A.
影响因子：
--
作者：
Hao L;Zou Z;Tian H;Zhang Y;Zhou H;Liu L
通讯作者：
Liu L
影响因子：
8.3
作者：
Leker, Ronen R.;Soldner, Frank;McKay, Ronald D. G.
通讯作者：
McKay, Ronald D. G.