Live imaging of mouse endogenous neural progenitors migrating in response to an induced tumor.
Live imaging of mouse endogenous neural progenitors migrating in response to an induced tumor.
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DOI：
10.1371/journal.pone.0044466
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Silva A
中科院分区：
文献类型：
--
作者：
Elvira G;García I;Benito M;Gallo J;Desco M;Penadés S;Garcia-Sanz JA;Silva A
成人神经发生仅限于特定的大脑区域。虽然参与嗅觉功能的中间神经元的持续供应，但神经前体在脑损伤修复中的作用仍然是一个悬而未决的问题。为了在体内鉴定向脑损伤部位迁移的内源性神经前体细胞，将识别成神经细胞上的细胞表面抗原的单克隆抗体Nilo 2偶联至磁性糖纳米颗粒（mGNP）。通过活体动物的磁共振成像，Nilo 2-mGNP复合物允许在体内鉴定内源性神经前体在它们的小生境，以及它们快速（在数小时内）有序地迁移到病变部位（诱导的脑肿瘤）。有趣的是，神经母细胞向损伤部位的快速迁移是一个可能被用来精确定位神经退行性疾病早期损伤事件的特征。此外，它可能有助于研究再生机制，通过激活内源性神经细胞前体。一种类似的方法，将磁性糖纳米颗粒与适当的抗体结合，可以用于标记生物体内的其他小细胞亚群，跟踪它们的迁移，定位干细胞壁龛，癌症干细胞甚至跟踪转移细胞。
Adult neurogenesis is restricted to specific brain regions. Although involved in the continuous supply of interneurons for the olfactory function, the role of neural precursors in brain damage-repair remains an open question. Aiming to in vivo identify endogenous neural precursor cells migrating towards a brain damage site, the monoclonal antibody Nilo2 recognizing cell surface antigens on neuroblasts, was coupled to magnetic glyconanoparticles (mGNPs). The Nilo2-mGNP complexes allowed, by magnetic resonance imaging in living animals, the in vivo identification of endogenous neural precursors at their niche, as well as their migration to a lesion site (induced brain tumor), which was fast (within hours) and orderly. Interestingly, the rapid migration of neuroblasts towards a damage site is a characteristic that might be exploited to precisely localize early damage events in neurodegenerative diseases. In addition, it might facilitate the study of regenerative mechanisms through the activation of endogenous neural cell precursors. A similar approach, combining magnetic glyconanoparticles linked to appropriate antibodies could be applied to flag other small cell subpopulations within the organism, track their migration, localize stem cell niches, cancer stem cells or even track metastatic cells.
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影响因子：
3.3
作者：
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通讯作者：
Bulte, Jeff W. M.
影响因子：
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作者：
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作者：
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通讯作者：
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作者：
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通讯作者：
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