Quality of corneal lamellar cuts quantified using atomic force microscopy.
Quality of corneal lamellar cuts quantified using atomic force microscopy.
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DOI：
10.1016/j.jcrs.2012.07.040
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发表时间：
2013-01
影响因子：
2.8
通讯作者：
Yoo SH
中科院分区：
文献类型：
--
作者：
Ziebarth NM;Dias J;Hürmeriç V;Shousha MA;Yau CB;Moy VT;Culbertson WW;Yoo SH
旨在使用原子力显微镜（AFM）量化飞秒激光进行的层状解剖的切割质量。美国佛罗里达迈阿密，迈阿密大学米勒医学院，巴斯科姆帕尔默眼科研究所。实验研究。实验在3对人尸眼上进行。通过将地球仪角膜侧朝下放置在25%葡聚糖中24小时，使角膜变薄至生理水平。通过向玻璃体腔内注射平衡盐溶液将眼睛重新充气至正常压力。将眼睛置于保持器中，去除上皮，并用Visumax飞秒激光切割眼睛。每对右眼的能量水平为180 nJ，左眼为340 nJ。切割深度为200 μm、300 μm和400 μm，每对眼睛的切割深度保持不变。然后进行12.0 mm环钻术。将层状表面的前部置于平衡盐溶液中并用AFM成像。作为对照，将后表面置于2%福尔马林中，并使用环境扫描电子显微镜（SEM）进行成像。四个定量参数（均方根偏差，平均偏差，偏度，峰度）计算从AFM图像。从原子力显微镜观察，300 μm的低能切割是最平滑的。在环境SEM图像中定性地观察到类似的结果。原子力显微镜提供了关于使用飞秒激光进行的板层剥离质量的定量信息，这有助于优化屈光和板层角膜移植手术中的患者结局。
To quantify the cut quality of lamellar dissections made with the femtosecond laser using atomic force microscopy (AFM). Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, USA. Experimental study. Experiments were performed on 3 pairs of human cadaver eyes. The cornea was thinned to physiologic levels by placing the globe, cornea side down, in 25% dextran for 24 hours. The eyes were reinflated to normal pressures by injecting a balanced salt solution into the vitreous cavity. The eyes were placed in a holder, the epithelium was removed, and the eyes were cut with a Visumax femtosecond laser. The energy level was 180 nJ for the right eye and 340 nJ for the left eye of each pair. The cut depths were 200 μm, 300 μm, and 400 μm, with the cut depth maintained for both eyes of each pair. A 12.0 mm trephination was then performed. The anterior portion of the lamellar surface was placed in a balanced salt solution and imaged with AFM. As a control, the posterior surface was placed in 2% formalin and imaged with environmental scanning electron microscopy (SEM). Four quantitative parameters (root-mean-square deviation, average deviation, skewness, kurtosis) were calculated from the AFM images. From AFM, the 300 μm low-energy cuts were the smoothest. Similar results were seen qualitatively in the environmental SEM images. Atomic force microscopy provided quantitative information on the quality of lamellar dissections made using a femtosecond laser, which is useful in optimizing patient outcomes in refractive and lamellar keratoplasty surgeries.
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